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Assay Detail

CHEMBL3756508

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of Escherichia coli beta-galactosidase assessed as p-nitrophenyl-beta-D-galactopyranoside substrate hydrolysis by UV/Vis spectroscopy
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-galactosidase (CHEMBL1293264)
Type SINGLE PROTEIN
Organism Escherichia coli

Publication

Illuminating the binding interactions of galactonoamidines during the inhibition of β-galactosidase (E. coli).
Fan QH, Pickens JB, Striegler S, Gervaise CD.
Bioorg Med Chem (2016) 24 : 661 -671
PubMed 26740154 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 8.50 10.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3359672 1 10.22
CHEMBL3752112 1 8.89
CHEMBL3753015 1 7.50
CHEMBL3754250 1 7.38

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3359672 Ki = 0.06 nM 10.22
CHEMBL3752112 Ki = 1.3 nM 8.89
CHEMBL3753015 Ki = 32.0 nM 7.50
CHEMBL3754250 Ki = 42.0 nM 7.38