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Assay Detail

CHEMBL3757559

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ASCT2-mediated glutamine transport in human HEK293 cells using [3H]-glutamine after 15 mins by scintillation counting
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neutral amino acid transporter B(0) (CHEMBL3562162)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

2-Amino-4-bis(aryloxybenzyl)aminobutanoic acids: A novel scaffold for inhibition of ASCT2-mediated glutamine transport.
Schulte ML, Khodadadi AB, Cuthbertson ML, Smith JA, Manning HC.
Bioorg Med Chem Lett (2016) 26 : 1044 -1047
PubMed 26750251 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 4.62 5.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3754498 1 5.14
CHEMBL3752210 1 5.10
CHEMBL3754053 1 5.02
CHEMBL3752483 1 4.94
CHEMBL3753592 1 4.92
CHEMBL3754059 1 4.86
CHEMBL3753993 1 4.76
CHEMBL3753404 1 4.75
CHEMBL3753173 1 4.59
CHEMBL3752943 1 4.58
CHEMBL3752249 1 4.47
CHEMBL3754369 1 4.47
CHEMBL3752316 1 4.40
CHEMBL3752103 1 4.32
CHEMBL3753808 1 4.29
CHEMBL3753869 1 4.24
CHEMBL3752494 1 4.22
CHEMBL3754415 1 4.16
CHEMBL6251 1 -
CHEMBL3752700 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3754498 IC50 = 7200.0 nM 5.14
CHEMBL3752210 IC50 = 7900.0 nM 5.10
CHEMBL3754053 IC50 = 9600.0 nM 5.02
CHEMBL3752483 IC50 = 11500.0 nM 4.94
CHEMBL3753592 IC50 = 11900.0 nM 4.92
CHEMBL3754059 IC50 = 13700.0 nM 4.86
CHEMBL3753993 IC50 = 17500.0 nM 4.76
CHEMBL3753404 IC50 = 17600.0 nM 4.75
CHEMBL3753173 IC50 = 25600.0 nM 4.59
CHEMBL3752943 IC50 = 26000.0 nM 4.58
CHEMBL3752249 IC50 = 33800.0 nM 4.47
CHEMBL3754369 IC50 = 33700.0 nM 4.47
CHEMBL3752316 IC50 = 40100.0 nM 4.40
CHEMBL3752103 IC50 = 48300.0 nM 4.32
CHEMBL3753808 IC50 = 50800.0 nM 4.29
CHEMBL3753869 IC50 = 57200.0 nM 4.24
CHEMBL3752494 IC50 = 59600.0 nM 4.22
CHEMBL3754415 IC50 = 69100.0 nM 4.16
CHEMBL6251 IC50 = 1200000.0 nM -
CHEMBL3752700 IC50 = 141700.0 nM -