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Assay Detail

CHEMBL3757620

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of JMJD3 (unknown origin) using biotinylated H3K27me3 peptide as substrate after 1 hr by AlphaLISA assay
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysine-specific demethylase 6B (CHEMBL1938211)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and discovery of new pyrimidine coupled nitrogen aromatic rings as chelating groups of JMJD3 inhibitors.
Hu J, Wang X, Chen L, Huang M, Tang W, Zuo J, Liu YC, Shi Z, Liu R, Shen J, Xiong B.
Bioorg Med Chem Lett (2016) 26 : 721 -725
PubMed 26776360 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 6.00 6.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3753391 1 6.82
CHEMBL3188597 1 6.82
CHEMBL3752893 1 6.70
CHEMBL3752179 1 6.68
CHEMBL3753740 1 6.57
CHEMBL3752769 1 6.31
CHEMBL3752835 1 6.26
CHEMBL3752143 1 6.22
CHEMBL3752551 1 6.13
CHEMBL3753449 1 6.12
CHEMBL3752294 1 6.05
CHEMBL3752343 1 6.03
CHEMBL3753199 1 6.00
CHEMBL3753326 1 6.00
CHEMBL3753893 1 5.92
CHEMBL3754078 1 5.92
CHEMBL3752967 1 5.89
CHEMBL3752156 1 5.85
CHEMBL3753356 1 5.85
CHEMBL3753787 1 5.82
CHEMBL3752995 1 5.68
CHEMBL3752006 1 5.68
CHEMBL3754555 1 5.66
CHEMBL3753303 1 5.58
CHEMBL3754777 1 5.24
CHEMBL3754383 1 5.21
CHEMBL3754052 1 5.00
CHEMBL3753122 1 -
CHEMBL3752327 1 -
CHEMBL3752471 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3753391 IC50 = 150.0 nM 6.82
CHEMBL3188597 IC50 = 150.0 nM 6.82
CHEMBL3752893 IC50 = 200.0 nM 6.70
CHEMBL3752179 IC50 = 210.0 nM 6.68
CHEMBL3753740 IC50 = 270.0 nM 6.57
CHEMBL3752769 IC50 = 490.0 nM 6.31
CHEMBL3752835 IC50 = 550.0 nM 6.26
CHEMBL3752143 IC50 = 600.0 nM 6.22
CHEMBL3752551 IC50 = 740.0 nM 6.13
CHEMBL3753449 IC50 = 750.0 nM 6.12
CHEMBL3752294 IC50 = 900.0 nM 6.05
CHEMBL3752343 IC50 = 940.0 nM 6.03
CHEMBL3753199 IC50 = 990.0 nM 6.00
CHEMBL3753326 IC50 = 1000.0 nM 6.00
CHEMBL3753893 IC50 = 1200.0 nM 5.92
CHEMBL3754078 IC50 = 1200.0 nM 5.92
CHEMBL3752967 IC50 = 1300.0 nM 5.89
CHEMBL3752156 IC50 = 1400.0 nM 5.85
CHEMBL3753356 IC50 = 1400.0 nM 5.85
CHEMBL3753787 IC50 = 1500.0 nM 5.82
CHEMBL3752995 IC50 = 2100.0 nM 5.68
CHEMBL3752006 IC50 = 2100.0 nM 5.68
CHEMBL3754555 IC50 = 2200.0 nM 5.66
CHEMBL3753303 IC50 = 2600.0 nM 5.58
CHEMBL3754777 IC50 = 5800.0 nM 5.24
CHEMBL3754383 IC50 = 6100.0 nM 5.21
CHEMBL3754052 IC50 = 10000.0 nM 5.00
CHEMBL3753122 IC50 > 20000.0 nM -
CHEMBL3752327 IC50 > 20000.0 nM -
CHEMBL3752471 IC50 > 100000.0 nM -