Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3757927

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at human S1P1 receptor expressed in EDG1-bla U2OS cells incubated for 18 hrs prior to GenBlazer substrate addition by beta-arrestin recruitment assay
ChEMBL ↗
55
Total Activities
55
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type U2OS
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sphingosine 1-phosphate receptor 1 (CHEMBL4333) ↗
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Late-stage optimization of a tercyclic class of S1P3-sparing, S1P1 receptor agonists.
Horan JC, Kuzmich D, Liu P, DiSalvo D, Lord J, Mao C, Hopkins TD, Yu H, Harcken C, Betageri R, Hill-Drzewi M, Patenaude L, Patel M, Fletcher K, Terenzzio D, Linehan B, Xia H, Patel M, Studwell D, Miller C, Hickey E, Levin JI, Smith D, Kemper RA, Modis LK, Bannen LC, Chan DS, Mac MB, Ng S, Wang Y, Xu W, Lemieux RM.
Bioorg Med Chem Lett (2016) 26 : 466 -471
PubMed 26687487 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 55 7.83 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3753584 — — 1 10.00
CHEMBL3753943 — — 1 9.70
CHEMBL366208 FTY720-P -1.0 1 9.40
CHEMBL3754163 — — 1 9.30
CHEMBL3753602 — — 1 9.30
CHEMBL3754018 — — 1 9.22
CHEMBL3751872 — — 1 9.10
CHEMBL3754270 — — 1 9.10
CHEMBL3754653 — — 1 9.10
CHEMBL3753209 — — 1 9.10
CHEMBL3752968 — — 1 8.85
CHEMBL3752339 — — 1 8.72
CHEMBL3752473 — — 1 8.60
CHEMBL3753059 — — 1 8.55
CHEMBL225155 — — 1 8.55
CHEMBL3752499 — — 1 8.46
CHEMBL3753931 — — 1 8.40
CHEMBL3752984 — — 1 8.39
CHEMBL3754035 — — 1 8.39
CHEMBL3752436 — — 1 8.22
CHEMBL3753881 — — 1 8.14
CHEMBL314854 FINGOLIMOD 4.0 1 8.14
CHEMBL3753282 — — 1 8.10
CHEMBL3753300 — — 1 7.96
CHEMBL3751895 — — 1 7.89
CHEMBL3752726 — — 1 7.89
CHEMBL3754266 — — 1 7.85
CHEMBL3753062 — — 1 7.75
CHEMBL3754355 — — 1 7.75
CHEMBL3753448 — — 1 7.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3753584 — EC50 = 0.1 nM 10.00
CHEMBL3753943 — EC50 = 0.2 nM 9.70
CHEMBL366208 FTY720-P EC50 = 0.4 nM 9.40
CHEMBL3754163 — EC50 = 0.5 nM 9.30
CHEMBL3753602 — EC50 = 0.5 nM 9.30
CHEMBL3754018 — EC50 = 0.6 nM 9.22
CHEMBL3751872 — EC50 = 0.8 nM 9.10
CHEMBL3754270 — EC50 = 0.8 nM 9.10
CHEMBL3754653 — EC50 = 0.8 nM 9.10
CHEMBL3753209 — EC50 = 0.8 nM 9.10
CHEMBL3752968 — EC50 = 1.4 nM 8.85
CHEMBL3752339 — EC50 = 1.9 nM 8.72
CHEMBL3752473 — EC50 = 2.5 nM 8.60
CHEMBL3753059 — EC50 = 2.8 nM 8.55
CHEMBL225155 — EC50 = 2.8 nM 8.55
CHEMBL3752499 — EC50 = 3.5 nM 8.46
CHEMBL3753931 — EC50 = 4.0 nM 8.40
CHEMBL3752984 — EC50 = 4.1 nM 8.39
CHEMBL3754035 — EC50 = 4.1 nM 8.39
CHEMBL3752436 — EC50 = 6.0 nM 8.22
CHEMBL3753881 — EC50 = 7.3 nM 8.14
CHEMBL314854 FINGOLIMOD EC50 = 7.2 nM 8.14
CHEMBL3753282 — EC50 = 7.9 nM 8.10
CHEMBL3753300 — EC50 = 11.0 nM 7.96
CHEMBL3752726 — EC50 = 13.0 nM 7.89
CHEMBL3751895 — EC50 = 13.0 nM 7.89
CHEMBL3754266 — EC50 = 14.0 nM 7.85
CHEMBL3753062 — EC50 = 18.0 nM 7.75
CHEMBL3754355 — EC50 = 18.0 nM 7.75
CHEMBL3753448 — EC50 = 20.0 nM 7.70
CHEMBL3752660 — EC50 = 32.0 nM 7.50
CHEMBL3753190 — EC50 = 32.0 nM 7.50
CHEMBL3752005 — EC50 = 37.0 nM 7.43
CHEMBL3753609 — EC50 = 42.0 nM 7.38
CHEMBL3753275 — EC50 = 49.0 nM 7.31
CHEMBL3753479 — EC50 = 50.0 nM 7.30
CHEMBL3752990 — EC50 = 62.0 nM 7.21
CHEMBL3753596 — EC50 = 63.0 nM 7.20
CHEMBL3754731 — EC50 = 67.0 nM 7.17
CHEMBL3752093 — EC50 = 86.0 nM 7.07
CHEMBL3754354 — EC50 = 100.0 nM 7.00
CHEMBL3753049 — EC50 = 105.0 nM 6.98
CHEMBL3752590 — EC50 = 120.0 nM 6.92
CHEMBL3752137 — EC50 = 130.0 nM 6.89
CHEMBL3754020 — EC50 = 147.0 nM 6.83
CHEMBL3753177 — EC50 = 150.0 nM 6.82
CHEMBL3752350 — EC50 = 160.0 nM 6.80
CHEMBL3753198 — EC50 = 200.0 nM 6.70
CHEMBL3754430 — EC50 = 250.0 nM 6.60
CHEMBL3753255 — EC50 = 300.0 nM 6.52