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Assay Detail

CHEMBL3758022

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]-DTyr6-His5-GnRH from human type 1 GnRH receptor expressed in HEK293 cell membranes after 16 to 19 hrs by gamma counting analysis
13
Total Activities
7
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Gonadotropin-releasing hormone receptor (CHEMBL1855)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel peptidomimetics related to gonadotropin-releasing hormone (GnRH)
Gelain A, Rizzi L, Legnani L, Pacini A, Spyridaki K, Karageorgos V, Liapakis G, Villa S
Medchemcomm (2015) 6 : 1656 -1665
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 6.83 9.54
Activity 6 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1201199 LEUPROLIDE 4.0 1 9.54
CHEMBL4299455 2 6.72
CHEMBL4299432 2 6.55
CHEMBL4299453 2 6.39
CHEMBL3753755 2 6.26
CHEMBL3754384 2 6.23
CHEMBL3754120 2 6.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1201199 LEUPROLIDE IC50 = 0.2884 nM 9.54
CHEMBL4299455 IC50 = 190.55 nM 6.72
CHEMBL4299432 IC50 = 281.84 nM 6.55
CHEMBL4299453 IC50 = 407.38 nM 6.39
CHEMBL3753755 IC50 = 549.54 nM 6.26
CHEMBL3754384 IC50 = 588.84 nM 6.23
CHEMBL3754120 IC50 = 724.44 nM 6.14
CHEMBL3754384 Activity - -
CHEMBL3753755 Activity - -
CHEMBL3754120 Activity - -
CHEMBL4299455 Activity - -
CHEMBL4299432 Activity - -
CHEMBL4299453 Activity - -