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Assay Detail

CHEMBL3760352

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Negative allosteric modulator activity at human CB1R expressed in CHO-K1 cells assessed as inhibition of CP55,940-induced beta-arrestin recruitment preincubated for 30 mins followed by CP55,940 addition measured for 90 mins by PathHunter assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cannabinoid receptor 1 (CHEMBL218)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel Electrophilic and Photoaffinity Covalent Probes for Mapping the Cannabinoid 1 Receptor Allosteric Site(s).
Kulkarni PM, Kulkarni AR, Korde A, Tichkule RB, Laprairie RB, Denovan-Wright EM, Zhou H, Janero DR, Zvonok N, Makriyannis A, Cascio MG, Pertwee RG, Thakur GA.
J Med Chem (2016) 59 : 44 -60
PubMed 26529344 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 9 7.52 8.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3758877 1 8.68
CHEMBL3341861 1 8.42
CHEMBL1553629 1 8.04
CHEMBL3758325 1 7.55
CHEMBL3758817 1 7.55
CHEMBL3759542 1 7.37
CHEMBL3758424 1 7.19
CHEMBL3758782 1 6.68
CHEMBL3758973 1 6.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3758877 EC50 = 2.09 nM 8.68
CHEMBL3341861 EC50 = 3.84 nM 8.42
CHEMBL1553629 EC50 = 9.05 nM 8.04
CHEMBL3758325 EC50 = 28.2 nM 7.55
CHEMBL3758817 EC50 = 28.2 nM 7.55
CHEMBL3759542 EC50 = 42.9 nM 7.37
CHEMBL3758424 EC50 = 64.0 nM 7.19
CHEMBL3758782 EC50 = 209.0 nM 6.68
CHEMBL3758973 EC50 = 683.0 nM 6.17