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Assay Detail

CHEMBL3762220

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-tagged recombinant human CDK2/cyclin E expressed in insect SF-9 cells after 10 mins by scintillation counting analysis in presence of [gamma-33P]-ATP
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Cyclin-dependent kinase 2/cyclin E1 (CHEMBL1907605)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Bis-isatin hydrazones with novel linkers: Synthesis and biological evaluation as cytotoxic agents.
Ibrahim HS, Abou-Seri SM, Ismail NS, Elaasser MM, Aly MH, Abdel-Aziz HA.
Eur J Med Chem (2016) 108 : 415 -422
PubMed 26706352 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.00 6.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL599552 INDIGO 3.0 1 6.00
CHEMBL1231469 1 6.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL599552 INDIGO IC50 = 1000.0 nM 6.00
CHEMBL1231469 IC50 = 1000.0 nM 6.00