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Assay Detail

CHEMBL3766652

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of JAK1 (unknown origin) using Ulight-JAK1 substrate peptide assessed as reduction in ATP-dependent substrate phosphorylation after 45 mins by TR-FRET assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK1 (CHEMBL2835)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Design and Synthesis of 3-Amino-1,5-dihydro-4H-pyrazolopyridin-4-one Derivatives as Tyrosine Kinase 2 Inhibitors.
Yogo T, Nagamiya H, Seto M, Sasaki S, Shih-Chung H, Ohba Y, Tokunaga N, Lee GN, Rhim CY, Yoon CH, Cho SY, Skene R, Yamamoto S, Satou Y, Kuno M, Miyazaki T, Nakagawa H, Okabe A, Marui S, Aso K, Yoshida M.
J Med Chem (2016) 59 : 733 -749
PubMed 26701356 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 7.13 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2105759 BARICITINIB 4.0 1 9.00
CHEMBL3763991 1 8.16
CHEMBL3763252 1 7.82
CHEMBL3765822 1 7.80
CHEMBL3763213 1 7.80
CHEMBL3764277 1 7.62
CHEMBL3764030 1 7.52
CHEMBL3764463 1 7.19
CHEMBL3765517 1 7.17
CHEMBL3764637 1 7.10
CHEMBL3764167 1 7.05
CHEMBL3765131 1 7.04
CHEMBL3764383 1 7.00
CHEMBL3763864 1 6.89
CHEMBL3763697 1 6.64
CHEMBL3763184 1 6.58
CHEMBL3763289 1 6.43
CHEMBL498859 1 6.31
CHEMBL3763310 1 6.00
CHEMBL3763833 1 5.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2105759 BARICITINIB IC50 = 0.99 nM 9.00
CHEMBL3763991 IC50 = 6.9 nM 8.16
CHEMBL3763252 IC50 = 15.0 nM 7.82
CHEMBL3765822 IC50 = 16.0 nM 7.80
CHEMBL3763213 IC50 = 16.0 nM 7.80
CHEMBL3764277 IC50 = 24.0 nM 7.62
CHEMBL3764030 IC50 = 30.0 nM 7.52
CHEMBL3764463 IC50 = 65.0 nM 7.19
CHEMBL3765517 IC50 = 67.0 nM 7.17
CHEMBL3764637 IC50 = 79.0 nM 7.10
CHEMBL3764167 IC50 = 90.0 nM 7.05
CHEMBL3765131 IC50 = 91.0 nM 7.04
CHEMBL3764383 IC50 = 100.0 nM 7.00
CHEMBL3763864 IC50 = 130.0 nM 6.89
CHEMBL3763697 IC50 = 230.0 nM 6.64
CHEMBL3763184 IC50 = 260.0 nM 6.58
CHEMBL3763289 IC50 = 370.0 nM 6.43
CHEMBL498859 IC50 = 490.0 nM 6.31
CHEMBL3763310 IC50 = 1000.0 nM 6.00
CHEMBL3763833 IC50 = 3100.0 nM 5.51