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Assay Detail

CHEMBL3768082

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human KM12 cells after 48 hrs by sulforhodamine B assay
6
Total Activities
2
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type KM12
Confidence 1 — Organism
Curated By Autocuration

Target

KM12 (CHEMBL614709)
Type CELL-LINE
Organism Homo sapiens

Publication

3,5-Bis(3-alkylaminomethyl-4-hydroxybenzylidene)-4-piperidones: A Novel Class of Potent Tumor-Selective Cytotoxins.
Karki SS, Das U, Umemura N, Sakagami H, Iwamoto S, Kawase M, Balzarini J, De Clercq E, Dimmock SG, Dimmock JR.
J Med Chem (2016) 59 : 763 -769
PubMed 26727215 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 5.42 6.00
TGI 2 - -
CC50 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3763325 3 6.00
CHEMBL185 FLUOROURACIL 4.0 3 4.83

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3763325 IC50 = 1000.0 nM 6.00
CHEMBL185 FLUOROURACIL IC50 = 14800.0 nM 4.83
CHEMBL3763325 TGI = 2190.0 nM -
CHEMBL185 FLUOROURACIL TGI > 1862000.0 nM -
CHEMBL3763325 CC50 = 4680.0 nM -
CHEMBL185 FLUOROURACIL CC50 > 2512000.0 nM -