Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3769086

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant Trypanosoma brucei PDEB1 using [3H]-cAMP as substrate after 15 mins by scintillation proximity assay
24
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Trypanosoma brucei
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Class 1 phosphodiesterase PDEB1 (CHEMBL2010636)
Type SINGLE PROTEIN
Organism Trypanosoma brucei

Publication

Synthesis and evaluation of analogs of the phenylpyridazinone NPD-001 as potent trypanosomal TbrPDEB1 phosphodiesterase inhibitors and in vitro trypanocidals.
Veerman J, van den Bergh T, Orrling KM, Jansen C, Cos P, Maes L, Chatelain E, Ioset JR, Edink EE, Tenor H, Seebeck T, de Esch I, Leurs R, Sterk GJ.
Bioorg Med Chem (2016) 24 : 1573 -1581
PubMed 26935942 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 6.80 8.50

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3765188 1 8.50
CHEMBL2326941 2 8.40
CHEMBL3763233 1 8.00
CHEMBL3765037 1 7.90
CHEMBL3764370 1 7.80
CHEMBL3765786 1 7.20
CHEMBL3765606 1 7.20
CHEMBL3765010 1 7.20
CHEMBL3763352 1 7.10
CHEMBL3765222 1 7.10
CHEMBL3764909 1 7.00
CHEMBL3765581 1 7.00
CHEMBL3765225 1 6.90
CHEMBL3765733 1 6.80
CHEMBL3763271 1 6.50
CHEMBL3763456 1 6.50
CHEMBL3763547 1 5.80
CHEMBL3764415 1 5.70
CHEMBL3764366 1 5.40
CHEMBL3763265 1 5.00
CHEMBL440878 1 4.90
CHEMBL3764675 1 4.00
CHEMBL3764466 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3765188 IC50 = 3.162 nM 8.50
CHEMBL2326941 IC50 = 4.0 nM 8.40
CHEMBL2326941 IC50 = 3.981 nM 8.40
CHEMBL3763233 IC50 = 10.0 nM 8.00
CHEMBL3765037 IC50 = 12.59 nM 7.90
CHEMBL3764370 IC50 = 15.85 nM 7.80
CHEMBL3765786 IC50 = 63.1 nM 7.20
CHEMBL3765606 IC50 = 63.1 nM 7.20
CHEMBL3765010 IC50 = 63.1 nM 7.20
CHEMBL3763352 IC50 = 79.43 nM 7.10
CHEMBL3765222 IC50 = 79.43 nM 7.10
CHEMBL3764909 IC50 = 100.0 nM 7.00
CHEMBL3765581 IC50 = 100.0 nM 7.00
CHEMBL3765225 IC50 = 125.89 nM 6.90
CHEMBL3765733 IC50 = 158.49 nM 6.80
CHEMBL3763271 IC50 = 316.23 nM 6.50
CHEMBL3763456 IC50 = 316.23 nM 6.50
CHEMBL3763547 IC50 = 1584.89 nM 5.80
CHEMBL3764415 IC50 = 1995.26 nM 5.70
CHEMBL3764366 IC50 = 3981.07 nM 5.40
CHEMBL3763265 IC50 = 10000.0 nM 5.00
CHEMBL440878 IC50 = 12589.25 nM 4.90
CHEMBL3764675 IC50 = 100000.0 nM 4.00
CHEMBL3764466 IC50 > 10000.0 nM -