Assay Detail
Binding
CHEMBL3772519
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Displacement of FITC-HIF-1alpha (556 to 574 residues) from PHD2 (181 to 426 residues) (unknown origin) after 60 mins by fluorescence polarization assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Affinity-Based Fluorescence Polarization Assay for High-Throughput Screening of Prolyl Hydroxylase 2 Inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 6.18 | 6.37 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL426560 | — | — | 1 | 6.37 |
| CHEMBL2338329 | ROXADUSTAT | 4.0 | 1 | 6.23 |
| CHEMBL3646118 | MOLIDUSTAT | 2.0 | 1 | 6.06 |
| CHEMBL3646215 | — | — | 1 | 6.05 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL426560 | — | IC50 | = | 424.2 | nM | 6.37 |
| CHEMBL2338329 | ROXADUSTAT | IC50 | = | 591.4 | nM | 6.23 |
| CHEMBL3646118 | MOLIDUSTAT | IC50 | = | 876.3 | nM | 6.06 |
| CHEMBL3646215 | — | IC50 | = | 890.4 | nM | 6.05 |