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Assay Detail

CHEMBL3774068

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of bovine liver beta-galactosidase using 4-nitrophenyl-beta-D-galactopyranoside as substrate preincubated up to 5 mins followed by substrate addition measured up to 10 mins
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-galactosidase (CHEMBL3482)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Synthesis of C-5a-chain extended derivatives of 4-epi-isofagomine: Powerful β-galactosidase inhibitors and low concentration activators of GM1-gangliosidosis-related human lysosomal β-galactosidase.
Thonhofer M, Weber P, Santana AG, Fischer R, Pabst BM, Paschke E, Schalli M, Stütz AE, Tschernutter M, Windischhofer W, Withers SG.
Bioorg Med Chem Lett (2016) 26 : 1438 -1442
PubMed 26838810 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 5.84 7.90

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3771185 1 7.90
CHEMBL3770764 1 5.40
CHEMBL3770736 1 5.05
CHEMBL1818433 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3771185 Ki = 12.7 nM 7.90
CHEMBL3770764 Ki = 4000.0 nM 5.40
CHEMBL3770736 Ki = 8940.0 nM 5.05
CHEMBL1818433 Ki = 10000.0 nM 5.00