Assay Detail
Binding
CHEMBL3776819
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of GST-tagged human LSD1 (172 to 833 residues) expressed in Escherichia coli C43(DE3) pLysS using biotinylated histone H3 mono methylated K4 peptide as substrate preincubated for 60 mins followed by substrate addition measured after 5 mins by time-resolved fluorescence assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Lysine-specific histone demethylase 1A (CHEMBL6136) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Cyclopropanamine Compounds and Use Thereof.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 6.67 | 6.75 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3775312 | — | — | 1 | 6.75 |
| CHEMBL3774654 | — | — | 1 | 6.68 |
| CHEMBL3775480 | — | — | 1 | 6.64 |
| CHEMBL3775248 | — | — | 1 | 6.62 |
| CHEMBL3775474 | — | — | 1 | - |
| CHEMBL3774809 | — | — | 1 | - |
| CHEMBL3775711 | — | — | 1 | - |
| CHEMBL3775351 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3775312 | — | IC50 | = | 180.0 | nM | 6.75 |
| CHEMBL3774654 | — | IC50 | = | 210.0 | nM | 6.68 |
| CHEMBL3775480 | — | IC50 | = | 230.0 | nM | 6.64 |
| CHEMBL3775248 | — | IC50 | = | 240.0 | nM | 6.62 |
| CHEMBL3775474 | — | IC50 | < | 100.0 | nM | - |
| CHEMBL3774809 | — | IC50 | < | 100.0 | nM | - |
| CHEMBL3775711 | — | IC50 | < | 100.0 | nM | - |
| CHEMBL3775351 | — | IC50 | < | 100.0 | nM | - |