Assay Detail
Functional
CHEMBL3777656
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by calcein AM staining based fluorescence assay
27
Total Activities
22
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | MCF7 |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Publication
5-Substituted 3-isopropyl-7-[4-(2-pyridyl)benzyl]amino-1(2)H-pyrazolo[4,3-d]pyrimidines with anti-proliferative activity as potent and selective inhibitors of cyclin-dependent kinases.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 22 | 6.61 | 7.68 |
| Activity | 5 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3774949 | — | — | 1 | 7.68 |
| CHEMBL3775792 | — | — | 1 | 7.62 |
| CHEMBL3774632 | — | — | 2 | 7.57 |
| CHEMBL3775502 | — | — | 1 | 7.29 |
| CHEMBL3774932 | — | — | 2 | 7.28 |
| CHEMBL3775677 | — | — | 1 | 7.23 |
| CHEMBL3775470 | — | — | 1 | 7.21 |
| CHEMBL3774979 | — | — | 1 | 6.88 |
| CHEMBL518800 | — | — | 1 | 6.80 |
| CHEMBL3775509 | — | — | 1 | 6.79 |
| CHEMBL3775467 | — | — | 1 | 6.71 |
| CHEMBL3775657 | — | — | 1 | 6.65 |
| CHEMBL3775223 | — | — | 1 | 6.53 |
| CHEMBL3775608 | — | — | 2 | 6.51 |
| CHEMBL3775606 | — | — | 1 | 6.34 |
| CHEMBL3774503 | — | — | 1 | 6.32 |
| CHEMBL3775681 | — | — | 1 | 6.28 |
| CHEMBL3775479 | — | — | 1 | 6.09 |
| CHEMBL3774434 | — | — | 2 | 5.92 |
| CHEMBL3775861 | — | — | 1 | 5.62 |
| CHEMBL3774636 | — | — | 2 | 5.28 |
| CHEMBL14762 | SELICICLIB | 2.0 | 1 | 4.91 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3774949 | — | IC50 | = | 21.0 | nM | 7.68 |
| CHEMBL3775792 | — | IC50 | = | 24.0 | nM | 7.62 |
| CHEMBL3774632 | — | IC50 | = | 27.0 | nM | 7.57 |
| CHEMBL3775502 | — | IC50 | = | 51.0 | nM | 7.29 |
| CHEMBL3774932 | — | IC50 | = | 53.0 | nM | 7.28 |
| CHEMBL3775677 | — | IC50 | = | 59.0 | nM | 7.23 |
| CHEMBL3775470 | — | IC50 | = | 62.0 | nM | 7.21 |
| CHEMBL3774979 | — | IC50 | = | 133.0 | nM | 6.88 |
| CHEMBL518800 | — | IC50 | = | 160.0 | nM | 6.80 |
| CHEMBL3775509 | — | IC50 | = | 163.0 | nM | 6.79 |
| CHEMBL3775467 | — | IC50 | = | 195.0 | nM | 6.71 |
| CHEMBL3775657 | — | IC50 | = | 225.0 | nM | 6.65 |
| CHEMBL3775223 | — | IC50 | = | 295.0 | nM | 6.53 |
| CHEMBL3775608 | — | IC50 | = | 310.0 | nM | 6.51 |
| CHEMBL3775606 | — | IC50 | = | 460.0 | nM | 6.34 |
| CHEMBL3774503 | — | IC50 | = | 474.0 | nM | 6.32 |
| CHEMBL3775681 | — | IC50 | = | 530.0 | nM | 6.28 |
| CHEMBL3775479 | — | IC50 | = | 819.0 | nM | 6.09 |
| CHEMBL3774434 | — | IC50 | = | 1190.0 | nM | 5.92 |
| CHEMBL3775861 | — | IC50 | = | 2385.0 | nM | 5.62 |
| CHEMBL3774636 | — | IC50 | = | 5300.0 | nM | 5.28 |
| CHEMBL14762 | SELICICLIB | IC50 | = | 12250.0 | nM | 4.91 |
| CHEMBL3774434 | — | Activity | - | — | - | |
| CHEMBL3774632 | — | Activity | - | — | - | |
| CHEMBL3774932 | — | Activity | - | — | - | |
| CHEMBL3775608 | — | Activity | - | — | - | |
| CHEMBL3774636 | — | Activity | - | — | - |