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Assay Detail

CHEMBL3778906

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Binding
Inhibition of mouse GAT1 expressed in HEK293 cells assessed as [3H]GABA uptake preincubated for 25 mins followed by [3H]GABA/unlabeled GABA addition measured after 4 mins by liquid scintillation counting analysis
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium- and chloride-dependent GABA transporter 1 (CHEMBL5445)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis of 4-substituted nipecotic acid derivatives and their evaluation as potential GABA uptake inhibitors.
Hellenbrand T, Höfner G, Wein T, Wanner KT.
Bioorg Med Chem (2016) 24 : 2072 -2096
PubMed 27039250 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 4.44 4.88

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL277498 NIPECOTIC ACID 1 4.88
CHEMBL3774606 1 4.61
CHEMBL3775938 1 4.61
CHEMBL3775303 1 4.59
CHEMBL3775848 1 4.58
CHEMBL3775466 1 4.46
CHEMBL3774755 1 4.43
CHEMBL3774662 1 4.43
CHEMBL3775354 1 4.39
CHEMBL3774577 1 4.22
CHEMBL3775673 1 4.19
CHEMBL3775725 1 4.17
CHEMBL3775361 1 4.12
CHEMBL3774505 1 -
CHEMBL3774496 1 -
CHEMBL3775515 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL277498 NIPECOTIC ACID IC50 = 13304.54 nM 4.88
CHEMBL3774606 IC50 = 24717.24 nM 4.61
CHEMBL3775938 IC50 = 24490.63 nM 4.61
CHEMBL3775303 IC50 = 25941.79 nM 4.59
CHEMBL3775848 IC50 = 26546.06 nM 4.58
CHEMBL3775466 IC50 = 34753.62 nM 4.46
CHEMBL3774755 IC50 = 36728.23 nM 4.43
CHEMBL3774662 IC50 = 37411.06 nM 4.43
CHEMBL3775354 IC50 = 40831.94 nM 4.39
CHEMBL3774577 IC50 = 60534.09 nM 4.22
CHEMBL3775673 IC50 = 64120.96 nM 4.19
CHEMBL3775725 IC50 = 66834.39 nM 4.17
CHEMBL3775361 IC50 = 75509.22 nM 4.12
CHEMBL3774505 IC50 = 106905.49 nM -
CHEMBL3774496 IC50 = 1150800.39 nM -
CHEMBL3775515 IC50 = 739605.28 nM -