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Assay Detail

CHEMBL3784644

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at mouse TRH-R2 expressed in HEK293 cells assessed as Ca2+ release by FLIPR assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Thyrotropin-releasing hormone receptor 2 (CHEMBL3600274)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis and biology of ring-modified l-Histidine containing thyrotropin-releasing hormone (TRH) analogues.
Meena CL, Thakur A, Nandekar PP, Sharma SS, Sangamwar AT, Jain R.
Eur J Med Chem (2016) 111 : 72 -83
PubMed 26854379 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 8.09 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3782037 1 10.00
CHEMBL3780645 1 9.40
CHEMBL3780746 1 9.34
CHEMBL1472 PROTIRELIN 4.0 1 9.30
CHEMBL3780633 1 9.00
CHEMBL3780373 1 8.89
CHEMBL3779931 1 8.84
CHEMBL3781773 1 7.57
CHEMBL3780549 1 7.52
CHEMBL3780802 1 7.42
CHEMBL3781522 1 7.06
CHEMBL3780191 1 6.69
CHEMBL3780836 1 6.44
CHEMBL3780248 1 5.72
CHEMBL3781024 1 -
CHEMBL3781873 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3782037 EC50 = 0.1 nM 10.00
CHEMBL3780645 EC50 = 0.4 nM 9.40
CHEMBL3780746 EC50 = 0.46 nM 9.34
CHEMBL1472 PROTIRELIN EC50 = 0.5 nM 9.30
CHEMBL3780633 EC50 = 1.0 nM 9.00
CHEMBL3780373 EC50 = 1.3 nM 8.89
CHEMBL3779931 EC50 = 1.46 nM 8.84
CHEMBL3781773 EC50 = 27.0 nM 7.57
CHEMBL3780549 EC50 = 30.0 nM 7.52
CHEMBL3780802 EC50 = 38.0 nM 7.42
CHEMBL3781522 EC50 = 87.0 nM 7.06
CHEMBL3780191 EC50 = 206.0 nM 6.69
CHEMBL3780836 EC50 = 360.0 nM 6.44
CHEMBL3780248 EC50 = 1910.0 nM 5.72
CHEMBL3781024 EC50 > 50000.0 nM -
CHEMBL3781873 EC50 > 50000.0 nM -