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Assay Detail

CHEMBL3788095

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat liver thioredoxin reductase after 60 mins by DTNB reduction assay in presence of NADPH
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Applying the designed multiple ligands approach to inhibit dihydrofolate reductase and thioredoxin reductase for anti-proliferative activity.
Ng HL, Chen S, Chew EH, Chui WK.
Eur J Med Chem (2016) 115 : 63 -74
PubMed 26994844 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.10 5.58

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3787235 1 5.58
CHEMBL3787681 1 5.38
CHEMBL3785320 1 5.27
CHEMBL3787397 1 5.00
CHEMBL7976 CHALCONE 1 4.97
CHEMBL3785981 1 4.90
CHEMBL104732 1 4.61
CHEMBL532909 1 -
CHEMBL34259 METHOTREXATE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3787235 IC50 = 2600.0 nM 5.58
CHEMBL3787681 IC50 = 4200.0 nM 5.38
CHEMBL3785320 IC50 = 5400.0 nM 5.27
CHEMBL3787397 IC50 = 10100.0 nM 5.00
CHEMBL7976 CHALCONE IC50 = 10800.0 nM 4.97
CHEMBL3785981 IC50 = 12600.0 nM 4.90
CHEMBL104732 IC50 = 24500.0 nM 4.61
CHEMBL532909 IC50 > 100000.0 nM -
CHEMBL34259 METHOTREXATE IC50 > 100000.0 nM -