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Assay Detail

CHEMBL3789067

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-{Nomega-[N-(4-propanoylaminobutyl)aminocarbonyl]}Arg-Arg-ProTyr-Ile-Leu-OH Tris(hydrotrifluoroacetate) from NTSR1 in human HT-29 cells after 2 hrs by liquid scintillation counting
16
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HT-29
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neurotensin receptor type 1 (CHEMBL4123)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Mimicking of Arginine by Functionalized N(ω)-Carbamoylated Arginine As a New Broadly Applicable Approach to Labeled Bioactive Peptides: High Affinity Angiotensin, Neuropeptide Y, Neuropeptide FF, and Neurotensin Receptor Ligands As Examples.
Keller M, Kuhn KK, Einsiedel J, Hübner H, Biselli S, Mollereau C, Wifling D, Svobodová J, Bernhardt G, Cabrele C, Vanderheyden PM, Gmeiner P, Buschauer A.
J Med Chem (2016) 59 : 1925 -1945
PubMed 26824643 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 9.23 9.85
IC50 6 8.78 9.39

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL415788 2 9.85
CHEMBL3786779 1 9.59
CHEMBL3785233 1 9.44
CHEMBL3787200 1 9.39
CHEMBL3786852 2 9.33
CHEMBL3786291 2 9.33
CHEMBL3786771 2 9.23
CHEMBL3040381 2 8.96
CHEMBL3787095 2 8.77
CHEMBL4299304 1 8.39

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL415788 Ki = 0.14 nM 9.85
CHEMBL3786779 Ki = 0.26 nM 9.59
CHEMBL3785233 Ki = 0.36 nM 9.44
CHEMBL3787200 Ki = 0.41 nM 9.39
CHEMBL415788 IC50 = 0.4074 nM 9.39
CHEMBL3786852 Ki = 0.47 nM 9.33
CHEMBL3786291 Ki = 0.47 nM 9.33
CHEMBL3786771 Ki = 0.59 nM 9.23
CHEMBL3040381 Ki = 1.1 nM 8.96
CHEMBL3786291 IC50 = 1.349 nM 8.87
CHEMBL3786852 IC50 = 1.349 nM 8.87
CHEMBL3787095 Ki = 1.7 nM 8.77
CHEMBL3786771 IC50 = 1.738 nM 8.76
CHEMBL3040381 IC50 = 3.162 nM 8.50
CHEMBL4299304 Ki = 4.1 nM 8.39
CHEMBL3787095 IC50 = 5.012 nM 8.30