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Assay Detail

CHEMBL3789140

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human recombinant 5-HT6 receptor expressed in CHO cells assessed as inhibition of agonist induced intracellular calcium levels by aequorin assay
32
Total Activities
32
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 6 (CHEMBL3371)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of N'-(arylsulfonyl)pyrazoline-1-carboxamidines by exploiting a novel interaction site in the 5-HT6 antagonistic binding pocket.
van Loevezijn A, Venhorst J, Iwema Bakker WI, Lange JH, de Looff W, Henzen R, de Vries J, van de Woestijne RP, den Hartog AP, Verhoog S, van der Neut MA, de Bruin NM, Kruse CG.
Bioorg Med Chem Lett (2016) 26 : 1605 -1611
PubMed 26876931 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 32 8.06 10.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3785512 1 10.30
CHEMBL3785100 1 9.50
CHEMBL3786752 1 9.10
CHEMBL3785453 1 9.00
CHEMBL3787525 1 8.80
CHEMBL3785231 1 8.60
CHEMBL3787201 1 8.50
CHEMBL3786973 1 8.40
CHEMBL3785626 1 8.40
CHEMBL3786693 1 8.30
CHEMBL3787508 1 8.30
CHEMBL1834226 1 8.30
CHEMBL3785259 1 8.10
CHEMBL3785431 1 8.00
CHEMBL3787014 1 8.00
CHEMBL3786087 1 7.90
CHEMBL1834345 1 7.80
CHEMBL1834225 1 7.80
CHEMBL1834234 1 7.70
CHEMBL3785864 1 7.60
CHEMBL1834352 1 7.20
CHEMBL3786703 1 7.20
CHEMBL3787246 1 7.10
CHEMBL3786563 1 7.00
CHEMBL3785278 1 6.90
CHEMBL3787388 1 5.80
CHEMBL3785894 1 -
CHEMBL3786951 1 -
CHEMBL3786432 1 -
CHEMBL3785105 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3785512 Kd = 0.05012 nM 10.30
CHEMBL3785100 Kd = 0.3162 nM 9.50
CHEMBL3786752 Kd = 0.7943 nM 9.10
CHEMBL3785453 Kd = 1.0 nM 9.00
CHEMBL3787525 Kd = 1.585 nM 8.80
CHEMBL3785231 Kd = 2.512 nM 8.60
CHEMBL3787201 Kd = 3.162 nM 8.50
CHEMBL3786973 Kd = 3.981 nM 8.40
CHEMBL3785626 Kd = 3.981 nM 8.40
CHEMBL3786693 Kd = 5.012 nM 8.30
CHEMBL3787508 Kd = 5.012 nM 8.30
CHEMBL1834226 Kd = 5.012 nM 8.30
CHEMBL3785259 Kd = 7.943 nM 8.10
CHEMBL3785431 Kd = 10.0 nM 8.00
CHEMBL3787014 Kd = 10.0 nM 8.00
CHEMBL3786087 Kd = 12.59 nM 7.90
CHEMBL1834345 Kd = 15.85 nM 7.80
CHEMBL1834225 Kd = 15.85 nM 7.80
CHEMBL1834234 Kd = 19.95 nM 7.70
CHEMBL3785864 Kd = 25.12 nM 7.60
CHEMBL1834352 Kd = 63.1 nM 7.20
CHEMBL3786703 Kd = 63.1 nM 7.20
CHEMBL3787246 Kd = 79.43 nM 7.10
CHEMBL3786563 Kd = 100.0 nM 7.00
CHEMBL3785278 Kd = 125.89 nM 6.90
CHEMBL3787388 Kd = 1584.89 nM 5.80
CHEMBL3785894 Kd > 1000.0 nM -
CHEMBL3786951 Kd > 1000.0 nM -
CHEMBL3786432 Kd > 1000.0 nM -
CHEMBL3785105 Kd > 1000.0 nM -
CHEMBL3786071 Kd > 1000.0 nM -
CHEMBL3785853 Kd > 1000.0 nM -