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Assay Detail

CHEMBL3789477

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of chymotrypsin like activity of human erythrocyte-derived 20S proteasome preincubated for 10 mins using Suc-LLVY-AMC as substrate after 60 mins by fluorescence assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proteasome Macropain subunit MB1 (CHEMBL4662)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and docking studies of novel dipeptidyl boronic acid proteasome inhibitors constructed from αα- and αβ-amino acids.
Shi J, Lei M, Wu W, Feng H, Wang J, Chen S, Zhu Y, Hu S, Liu Z, Jiang C.
Bioorg Med Chem Lett (2016) 26 : 1958 -1962
PubMed 26965867 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 8.44 8.99

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3786398 1 8.99
CHEMBL499361 1 8.92
CHEMBL499504 1 8.80
CHEMBL3785681 1 8.33
CHEMBL3786484 1 8.32
CHEMBL3785613 1 8.20
CHEMBL3787673 1 8.16
CHEMBL325041 BORTEZOMIB 3.0 1 8.15
CHEMBL3785317 1 8.07
CHEMBL3787060 1 -
CHEMBL3787687 1 -
CHEMBL3786571 1 -
CHEMBL3787573 1 -
CHEMBL3785411 1 -
CHEMBL3787320 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3786398 IC50 = 1.02 nM 8.99
CHEMBL499361 IC50 = 1.2 nM 8.92
CHEMBL499504 IC50 = 1.6 nM 8.80
CHEMBL3785681 IC50 = 4.69 nM 8.33
CHEMBL3786484 IC50 = 4.82 nM 8.32
CHEMBL3785613 IC50 = 6.31 nM 8.20
CHEMBL3787673 IC50 = 6.94 nM 8.16
CHEMBL325041 BORTEZOMIB IC50 = 7.09 nM 8.15
CHEMBL3785317 IC50 = 8.47 nM 8.07
CHEMBL3787060 IC50 - -
CHEMBL3787687 IC50 - -
CHEMBL3786571 IC50 - -
CHEMBL3787573 IC50 - -
CHEMBL3785411 IC50 - -
CHEMBL3787320 IC50 - -