Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3794752

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human N-terminal GST-tagged FLT3 cytoplasmic domain (564 to 993 residues) expressed in baculovirus expression system using 5-FAM-peptide 2 as substrate after 2 hrs by electrophoretic mobility shift assay in presence of ATP
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Receptor-type tyrosine-protein kinase FLT3 (CHEMBL1974)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of a potent and selective FLT3 kinase inhibitor by systematic expansion of a non-selective fragment-screening hit.
Nakano H, Hasegawa T, Imamura R, Saito N, Kojima H, Okabe T, Nagano T.
Bioorg Med Chem Lett (2016) 26 : 2370 -2374
PubMed 26995531 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 6.93 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3792850 1 8.00
CHEMBL3793843 1 7.47
CHEMBL3793804 1 7.38
CHEMBL3793847 1 7.37
CHEMBL3793427 1 7.34
CHEMBL3792973 1 7.25
CHEMBL3794211 1 7.21
CHEMBL3793695 1 7.05
CHEMBL3792878 1 7.02
CHEMBL3792955 1 6.97
CHEMBL3793933 1 6.96
CHEMBL3794503 1 6.95
CHEMBL3794507 1 6.55
CHEMBL3794415 1 6.51
CHEMBL1288651 1 6.45
CHEMBL508964 1 6.38
CHEMBL3793590 1 6.35
CHEMBL3792695 1 6.26
CHEMBL3793337 1 6.11
CHEMBL3793334 1 -
CHEMBL3792732 1 -
CHEMBL3794579 1 -
CHEMBL3793040 1 -
CHEMBL3794143 1 -
CHEMBL3792669 1 -
CHEMBL3792750 1 -
CHEMBL3792787 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3792850 IC50 = 10.0 nM 8.00
CHEMBL3793843 IC50 = 34.0 nM 7.47
CHEMBL3793804 IC50 = 42.0 nM 7.38
CHEMBL3793847 IC50 = 43.0 nM 7.37
CHEMBL3793427 IC50 = 46.0 nM 7.34
CHEMBL3792973 IC50 = 56.0 nM 7.25
CHEMBL3794211 IC50 = 61.0 nM 7.21
CHEMBL3793695 IC50 = 90.0 nM 7.05
CHEMBL3792878 IC50 = 95.0 nM 7.02
CHEMBL3792955 IC50 = 107.0 nM 6.97
CHEMBL3793933 IC50 = 110.0 nM 6.96
CHEMBL3794503 IC50 = 113.0 nM 6.95
CHEMBL3794507 IC50 = 281.0 nM 6.55
CHEMBL3794415 IC50 = 310.0 nM 6.51
CHEMBL1288651 IC50 = 352.0 nM 6.45
CHEMBL508964 IC50 = 417.0 nM 6.38
CHEMBL3793590 IC50 = 443.0 nM 6.35
CHEMBL3792695 IC50 = 552.0 nM 6.26
CHEMBL3793337 IC50 = 786.0 nM 6.11
CHEMBL3793334 IC50 > 2000.0 nM -
CHEMBL3792732 IC50 > 2000.0 nM -
CHEMBL3794579 IC50 > 2000.0 nM -
CHEMBL3793040 IC50 > 2000.0 nM -
CHEMBL3794143 IC50 > 2000.0 nM -
CHEMBL3792669 IC50 > 2000.0 nM -
CHEMBL3792750 IC50 > 2000.0 nM -
CHEMBL3792787 IC50 > 2000.0 nM -