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Assay Detail

CHEMBL3801876

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]Tyr-ovine-CRF from CRF1 receptor in rat frontal cortex homogenate after 2 hrs by gamma counting analysis
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Frontal cortex
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Corticotropin-releasing factor receptor 1 (CHEMBL4649)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and evaluation of carbamate and aryl ether substituted pyrazinones as corticotropin releasing factor-1 (CRF₁) receptor antagonists.
Ahuja VT, Hartz RA, Molski TF, Mattson GK, Lentz KA, Grace JE, Lodge NJ, Bronson JJ, Macor JE.
Bioorg Med Chem Lett (2016) 26 : 2184 -2187
PubMed 27020524 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 8.47 9.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL574852 1 9.54
CHEMBL3799870 1 9.13
CHEMBL44698 1 8.92
CHEMBL3797830 1 8.92
CHEMBL3799337 1 8.89
CHEMBL3797992 1 8.82
CHEMBL3798885 1 8.82
CHEMBL3799452 1 8.82
CHEMBL3800497 1 8.82
CHEMBL574436 1 8.82
CHEMBL3799036 1 8.80
CHEMBL3798004 1 8.80
CHEMBL3800413 1 8.77
CHEMBL3799825 1 8.77
CHEMBL3799784 1 8.72
CHEMBL3799560 1 8.33
CHEMBL3797551 1 8.31
CHEMBL3797368 1 8.28
CHEMBL3800125 1 8.28
CHEMBL3799801 1 8.17
CHEMBL3799211 1 8.17
CHEMBL3797955 1 8.11
CHEMBL3798497 1 8.04
CHEMBL3800523 1 8.00
CHEMBL3799417 1 8.00
CHEMBL3800035 1 7.85
CHEMBL3800457 1 7.17
CHEMBL3799741 1 7.06

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL574852 IC50 = 0.29 nM 9.54
CHEMBL3799870 IC50 = 0.74 nM 9.13
CHEMBL44698 IC50 = 1.2 nM 8.92
CHEMBL3797830 IC50 = 1.2 nM 8.92
CHEMBL3799337 IC50 = 1.3 nM 8.89
CHEMBL3797992 IC50 = 1.5 nM 8.82
CHEMBL3798885 IC50 = 1.5 nM 8.82
CHEMBL3799452 IC50 = 1.5 nM 8.82
CHEMBL3800497 IC50 = 1.5 nM 8.82
CHEMBL574436 IC50 = 1.5 nM 8.82
CHEMBL3799036 IC50 = 1.6 nM 8.80
CHEMBL3798004 IC50 = 1.6 nM 8.80
CHEMBL3800413 IC50 = 1.7 nM 8.77
CHEMBL3799825 IC50 = 1.7 nM 8.77
CHEMBL3799784 IC50 = 1.9 nM 8.72
CHEMBL3799560 IC50 = 4.7 nM 8.33
CHEMBL3797551 IC50 = 4.9 nM 8.31
CHEMBL3797368 IC50 = 5.3 nM 8.28
CHEMBL3800125 IC50 = 5.2 nM 8.28
CHEMBL3799801 IC50 = 6.8 nM 8.17
CHEMBL3799211 IC50 = 6.7 nM 8.17
CHEMBL3797955 IC50 = 7.7 nM 8.11
CHEMBL3798497 IC50 = 9.2 nM 8.04
CHEMBL3800523 IC50 = 10.0 nM 8.00
CHEMBL3799417 IC50 = 10.0 nM 8.00
CHEMBL3800035 IC50 = 14.0 nM 7.85
CHEMBL3800457 IC50 = 67.0 nM 7.17
CHEMBL3799741 IC50 = 88.0 nM 7.06