Assay Detail
Binding
CHEMBL3802801
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of N-(fluorescein-5-yl)-N'-(alpha-D-mannopyranosyloxyethyl)-thiocarbamide from Burkholderia cenocepacia J2315 BC2L-A expressed in Escherichia coli BL21 (DE3) cells incubated for 3 to 4 hrs by fluorescence polarization assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Burkholderia cenocepacia J2315 |
| Cell Type | BL21-DE3 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Lectin (CHEMBL3797020) ↗| Type | SINGLE PROTEIN |
| Organism | Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656/ NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315)) |
Publication
Development of a competitive binding assay for the Burkholderia cenocepacia lectin BC2L-A and structure activity relationship of natural and synthetic inhibitors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 13 | 4.92 | 5.54 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3800290 | — | — | 1 | 5.54 |
| CHEMBL195368 | — | — | 1 | 5.16 |
| CHEMBL3797837 | — | — | 1 | 5.13 |
| CHEMBL469448 | D-MANNOSE | 2.0 | 1 | 4.97 |
| CHEMBL3800313 | — | — | 1 | 4.89 |
| CHEMBL3799758 | — | — | 1 | 4.87 |
| CHEMBL3797852 | — | — | 1 | 4.86 |
| CHEMBL1172750 | — | — | 1 | 4.84 |
| CHEMBL3800386 | — | — | 1 | 4.72 |
| CHEMBL3797996 | — | — | 1 | 4.19 |
| CHEMBL3798825 | — | — | 1 | - |
| CHEMBL3797336 | — | — | 1 | - |
| CHEMBL6067643 | L-FUCOSE | 3.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3800290 | — | IC50 | = | 2900.0 | nM | 5.54 |
| CHEMBL195368 | — | IC50 | = | 7000.0 | nM | 5.16 |
| CHEMBL3797837 | — | IC50 | = | 7400.0 | nM | 5.13 |
| CHEMBL469448 | D-MANNOSE | IC50 | = | 10800.0 | nM | 4.97 |
| CHEMBL3800313 | — | IC50 | = | 13000.0 | nM | 4.89 |
| CHEMBL3799758 | — | IC50 | = | 13600.0 | nM | 4.87 |
| CHEMBL3797852 | — | IC50 | = | 13800.0 | nM | 4.86 |
| CHEMBL1172750 | — | IC50 | = | 14500.0 | nM | 4.84 |
| CHEMBL3800386 | — | IC50 | = | 19100.0 | nM | 4.72 |
| CHEMBL3797996 | — | IC50 | = | 64000.0 | nM | 4.19 |
| CHEMBL3798825 | — | IC50 | = | 104000.0 | nM | - |
| CHEMBL3797336 | — | IC50 | = | 116000.0 | nM | - |
| CHEMBL6067643 | L-FUCOSE | IC50 | = | 498000.0 | nM | - |