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Assay Detail

CHEMBL3802802

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to Burkholderia cenocepacia J2315 BC2L-A expressed in Escherichia coli BL21 (DE3) cells by microcalorimetry
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Burkholderia cenocepacia J2315
Cell Type BL21-DE3
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lectin (CHEMBL3797020)
Type SINGLE PROTEIN
Organism Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656/ NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))

Publication

Development of a competitive binding assay for the Burkholderia cenocepacia lectin BC2L-A and structure activity relationship of natural and synthetic inhibitors
Beshr G, Sommer R, Hauck D, Siebert DCB, Hofmann A, Imberty A, Titz A
Medchemcomm (2016) 7 : 519 -530
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 2 5.43 5.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL195368 1 5.57
CHEMBL469448 D-MANNOSE 2.0 1 5.29

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL195368 Kd = 2700.0 nM 5.57
CHEMBL469448 D-MANNOSE Kd = 5150.0 nM 5.29