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Assay Detail

CHEMBL3803372

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 125I-NDP-MSH from mouse MC4R expressed in HEK293 cells incubated for 1 hr by gamma counting analysis
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocortin receptor 4 (CHEMBL3719)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

An in Vitro and in Vivo Investigation of Bivalent Ligands That Display Preferential Binding and Functional Activity for Different Melanocortin Receptor Homodimers.
Lensing CJ, Freeman KT, Schnell SM, Adank DN, Speth RC, Haskell-Luevano C.
J Med Chem (2016) 59 : 3112 -3128
PubMed 26959173 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 7.37 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL441738 AFAMELANOTIDE 4.0 1 8.96
CHEMBL3798421 1 8.00
CHEMBL3798768 1 7.98
CHEMBL3799408 1 7.97
CHEMBL3798912 1 7.66
CHEMBL103817 1 7.58
CHEMBL4299619 1 7.48
CHEMBL3799955 1 7.27
CHEMBL3797690 1 7.08
CHEMBL264190 1 6.67
CHEMBL3799094 1 6.59
CHEMBL3799563 1 6.54
CHEMBL4299612 1 6.08

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL441738 AFAMELANOTIDE IC50 = 1.09 nM 8.96
CHEMBL3798421 IC50 = 9.9 nM 8.00
CHEMBL3798768 IC50 = 10.4 nM 7.98
CHEMBL3799408 IC50 = 10.7 nM 7.97
CHEMBL3798912 IC50 = 21.8 nM 7.66
CHEMBL103817 IC50 = 26.0 nM 7.58
CHEMBL4299619 IC50 = 33.0 nM 7.48
CHEMBL3799955 IC50 = 54.0 nM 7.27
CHEMBL3797690 IC50 = 83.0 nM 7.08
CHEMBL264190 IC50 = 214.0 nM 6.67
CHEMBL3799094 IC50 = 258.0 nM 6.59
CHEMBL3799563 IC50 = 292.0 nM 6.54
CHEMBL4299612 IC50 = 841.0 nM 6.08