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Assay Detail

CHEMBL3803380

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Functional
Agonist activity at mouse MC3R expressed in HEK293 cells assessed as cAMP accumulation incubated for 2 hrs by alphascreen assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocortin receptor 3 (CHEMBL4774)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

An in Vitro and in Vivo Investigation of Bivalent Ligands That Display Preferential Binding and Functional Activity for Different Melanocortin Receptor Homodimers.
Lensing CJ, Freeman KT, Schnell SM, Adank DN, Speth RC, Haskell-Luevano C.
J Med Chem (2016) 59 : 3112 -3128
PubMed 26959173 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 11 7.83 9.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL441738 AFAMELANOTIDE 4.0 1 9.62
CHEMBL214332 INTERMEDINE 2.0 1 9.12
CHEMBL3798421 1 8.00
CHEMBL4299619 1 7.70
CHEMBL3799955 1 7.64
CHEMBL3799094 1 7.57
CHEMBL3799563 1 7.51
CHEMBL405282 1 7.46
CHEMBL3797690 1 7.32
CHEMBL264190 1 7.26
CHEMBL4299612 1 6.97

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL441738 AFAMELANOTIDE EC50 = 0.24 nM 9.62
CHEMBL214332 INTERMEDINE EC50 = 0.76 nM 9.12
CHEMBL3798421 EC50 = 10.1 nM 8.00
CHEMBL4299619 EC50 = 20.2 nM 7.70
CHEMBL3799955 EC50 = 22.7 nM 7.64
CHEMBL3799094 EC50 = 27.2 nM 7.57
CHEMBL3799563 EC50 = 30.9 nM 7.51
CHEMBL405282 EC50 = 34.6 nM 7.46
CHEMBL3797690 EC50 = 47.4 nM 7.32
CHEMBL264190 EC50 = 55.5 nM 7.26
CHEMBL4299612 EC50 = 107.0 nM 6.97