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Assay Detail

CHEMBL3804575

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human Nav1.2 expressed in HEK293 cells preincubated for 40 mins followed by DiSBAC2 substrate addition measured after 90 mins by FRET assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium channel protein type 2 subunit alpha (CHEMBL4187)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Substituted Indazoles as Nav1.7 Blockers for the Treatment of Pain.
Frost JM, DeGoey DA, Shi L, Gum RJ, Fricano MM, Lundgaard GL, El-Kouhen OF, Hsieh GC, Neelands T, Matulenko MA, Daanen JF, Pai M, Ghoreishi-Haack N, Zhan C, Zhang XF, Kort ME.
J Med Chem (2016) 59 : 3373 -3391
PubMed 27015369 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.76 6.31

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL698 TETRACAINE 4.0 1 6.31
CHEMBL3798363 1 5.58
CHEMBL3798228 1 5.40
CHEMBL3798320 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL698 TETRACAINE IC50 = 490.0 nM 6.31
CHEMBL3798363 IC50 = 2600.0 nM 5.58
CHEMBL3798228 IC50 = 4000.0 nM 5.40
CHEMBL3798320 IC50 > 20000.0 nM -