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Assay Detail

CHEMBL3807564

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mouse full length GST-tagged BRAF V600E mutant assessed as reduction in human full length N-terminal His-tagged MEK1 phosphorylation pre-incubated for 1 hr before MEK1 addition and measured after 25 mins
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Design, biological evaluation and 3D QSAR studies of novel dioxin-containing triaryl pyrazoline derivatives as potential B-Raf inhibitors.
Yang YS, Yang B, Zou Y, Li G, Zhu HL.
Bioorg Med Chem (2016) 24 : 3052 -3061
PubMed 27238841 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 6.11 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1229517 VEMURAFENIB 4.0 1 7.52
CHEMBL3805508 1 7.40
CHEMBL553 ERLOTINIB 4.0 1 7.22
CHEMBL3806108 1 7.16
CHEMBL2146827 1 6.96
CHEMBL3805893 1 6.96
CHEMBL3805410 1 6.85
CHEMBL3805409 1 6.82
CHEMBL3805675 1 6.68
CHEMBL3805849 1 6.57
CHEMBL3805476 1 6.27
CHEMBL3804945 1 6.21
CHEMBL3805819 1 6.07
CHEMBL3805248 1 5.94
CHEMBL3805796 1 5.41
CHEMBL3804881 1 5.37
CHEMBL3805161 1 5.29
CHEMBL3804914 1 5.16
CHEMBL3805227 1 5.09
CHEMBL3804840 1 4.97
CHEMBL3805397 1 4.44
CHEMBL3805052 1 4.08
CHEMBL3805300 1 -
CHEMBL3805803 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1229517 VEMURAFENIB IC50 = 30.0 nM 7.52
CHEMBL3805508 IC50 = 40.0 nM 7.40
CHEMBL553 ERLOTINIB IC50 = 60.0 nM 7.22
CHEMBL3806108 IC50 = 70.0 nM 7.16
CHEMBL2146827 IC50 = 110.0 nM 6.96
CHEMBL3805893 IC50 = 110.0 nM 6.96
CHEMBL3805410 IC50 = 140.0 nM 6.85
CHEMBL3805409 IC50 = 150.0 nM 6.82
CHEMBL3805675 IC50 = 210.0 nM 6.68
CHEMBL3805849 IC50 = 270.0 nM 6.57
CHEMBL3805476 IC50 = 540.0 nM 6.27
CHEMBL3804945 IC50 = 610.0 nM 6.21
CHEMBL3805819 IC50 = 860.0 nM 6.07
CHEMBL3805248 IC50 = 1140.0 nM 5.94
CHEMBL3805796 IC50 = 3880.0 nM 5.41
CHEMBL3804881 IC50 = 4270.0 nM 5.37
CHEMBL3805161 IC50 = 5170.0 nM 5.29
CHEMBL3804914 IC50 = 6850.0 nM 5.16
CHEMBL3805227 IC50 = 8050.0 nM 5.09
CHEMBL3804840 IC50 = 10700.0 nM 4.97
CHEMBL3805397 IC50 = 36300.0 nM 4.44
CHEMBL3805052 IC50 = 83600.0 nM 4.08
CHEMBL3805300 IC50 = 118000.0 nM -
CHEMBL3805803 IC50 > 300000.0 nM -