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Assay Detail

CHEMBL3810823

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human BCR-ABL T315I mutant expressed in mouse BAF3 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type BaF3
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Bcr/Abl fusion protein (CHEMBL2096618)
Type CHIMERIC PROTEIN
Organism Homo sapiens

Publication

Rational Design, Synthesis, and Biological Evaluation of 7-Azaindole Derivatives as Potent Focused Multi-Targeted Kinase Inhibitors.
Daydé-Cazals B, Fauvel B, Singer M, Feneyrolles C, Bestgen B, Gassiot F, Spenlinhauer A, Warnault P, Van Hijfte N, Borjini N, Chevé G, Yasri A.
J Med Chem (2016) 59 : 3886 -3905
PubMed 27010810 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 8 9.13 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1171837 PONATINIB 4.0 1 9.52
CHEMBL3808884 1 8.74
CHEMBL5416410 DASATINIB 4.0 1 -
CHEMBL553 ERLOTINIB 4.0 1 -
CHEMBL535 SUNITINIB 4.0 1 -
CHEMBL941 IMATINIB 4.0 1 -
CHEMBL1336 SORAFENIB 4.0 1 -
CHEMBL1173655 AFATINIB 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1171837 PONATINIB EC50 = 0.3 nM 9.52
CHEMBL3808884 EC50 = 1.8 nM 8.74
CHEMBL5416410 DASATINIB EC50 > 1000.0 nM -
CHEMBL553 ERLOTINIB EC50 - -
CHEMBL535 SUNITINIB EC50 > 1000.0 nM -
CHEMBL941 IMATINIB EC50 > 1000.0 nM -
CHEMBL1336 SORAFENIB EC50 - -
CHEMBL1173655 AFATINIB EC50 - -