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Assay Detail

CHEMBL3811631

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant COX2 assessed as reduction in PGH2-derived PGF2alpha using arachidonic acid as substrate preincubated for 10 mins followed by substrate addition measured after 2 mins by enzyme immunoassay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin G/H synthase 2 (CHEMBL230)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Rational Design of Small Peptides for Optimal Inhibition of Cyclooxygenase-2: Development of a Highly Effective Anti-Inflammatory Agent.
Singh P, Kaur S, Kaur J, Singh G, Bhatti R.
J Med Chem (2016) 59 : 3920 -3934
PubMed 27019010 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 5.79 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL139 DICLOFENAC 4.0 1 7.70
CHEMBL118 CELECOXIB 4.0 1 7.40
CHEMBL3809863 1 7.30
CHEMBL3809156 1 7.22
CHEMBL3809842 1 7.10
CHEMBL3808627 1 7.00
CHEMBL3809958 1 6.70
CHEMBL3809120 1 6.52
CHEMBL3809116 1 6.40
CHEMBL3810167 1 6.30
CHEMBL3809154 1 6.00
CHEMBL3810057 1 5.70
CHEMBL3809328 1 5.70
CHEMBL3809760 1 5.52
CHEMBL3810258 1 5.10
CHEMBL3808663 1 5.10
CHEMBL3809922 1 5.00
CHEMBL3809099 1 4.72
CHEMBL3810371 1 4.70
CHEMBL3808516 1 4.66
CHEMBL3809163 1 4.55
CHEMBL3810231 1 4.26
CHEMBL3808774 1 4.25
CHEMBL3810027 1 4.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL139 DICLOFENAC IC50 = 20.0 nM 7.70
CHEMBL118 CELECOXIB IC50 = 40.0 nM 7.40
CHEMBL3809863 IC50 = 50.0 nM 7.30
CHEMBL3809156 IC50 = 60.0 nM 7.22
CHEMBL3809842 IC50 = 80.0 nM 7.10
CHEMBL3808627 IC50 = 100.0 nM 7.00
CHEMBL3809958 IC50 = 200.0 nM 6.70
CHEMBL3809120 IC50 = 300.0 nM 6.52
CHEMBL3809116 IC50 = 400.0 nM 6.40
CHEMBL3810167 IC50 = 500.0 nM 6.30
CHEMBL3809154 IC50 = 1000.0 nM 6.00
CHEMBL3810057 IC50 = 2000.0 nM 5.70
CHEMBL3809328 IC50 = 2000.0 nM 5.70
CHEMBL3809760 IC50 = 3000.0 nM 5.52
CHEMBL3810258 IC50 = 8000.0 nM 5.10
CHEMBL3808663 IC50 = 8000.0 nM 5.10
CHEMBL3809922 IC50 = 10000.0 nM 5.00
CHEMBL3809099 IC50 = 19000.0 nM 4.72
CHEMBL3810371 IC50 = 20000.0 nM 4.70
CHEMBL3808516 IC50 = 22000.0 nM 4.66
CHEMBL3809163 IC50 = 28000.0 nM 4.55
CHEMBL3810231 IC50 = 55000.0 nM 4.26
CHEMBL3808774 IC50 = 56000.0 nM 4.25
CHEMBL3810027 IC50 = 75000.0 nM 4.12