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Assay Detail

CHEMBL3812822

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of bovine testis hyaluronidase using hyaluronic acid as substrate preincubated for 20 mins followed by incubation with CaCl2 for 20 mins and substrate for 10 mins by colorimetric Morgan-Elson method
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Interpreting the behavior of concentration-response curves of hyaluronidase inhibitors under DMSO-perturbed assay conditions.
Tomohara K, Ito T, Onikata S, Furusawa K, Kato A, Adachi I.
Bioorg Med Chem Lett (2016) 26 : 3153 -3157
PubMed 27165854 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 4.86 5.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL441687 GLYCYRRHIZIN 2.0 1 5.07
CHEMBL3809846 1 4.64
CHEMBL485818 BAICALIN 1 -
CHEMBL6 INDOMETHACIN 4.0 1 -
CHEMBL74 CROMOLYN SODIUM 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL441687 GLYCYRRHIZIN IC50 = 8500.0 nM 5.07
CHEMBL3809846 IC50 = 23000.0 nM 4.64
CHEMBL485818 BAICALIN IC50 = 450000.0 nM -
CHEMBL6 INDOMETHACIN IC50 = 340000.0 nM -
CHEMBL74 CROMOLYN SODIUM IC50 = 330000.0 nM -