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Assay Detail

CHEMBL3817157

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of biotinylated ligand from recombinant His-tagged CBP (unknown origin) incubated for 10 mins by TR-FRET assay
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

CREB-binding protein (CHEMBL5747)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Fragment-Based Discovery of a Selective and Cell-Active Benzodiazepinone CBP/EP300 Bromodomain Inhibitor (CPI-637).
Taylor AM, Côté A, Hewitt MC, Pastor R, Leblanc Y, Nasveschuk CG, Romero FA, Crawford TD, Cantone N, Jayaram H, Setser J, Murray J, Beresini MH, de Leon Boenig G, Chen Z, Conery AR, Cummings RT, Dakin LA, Flynn EM, Huang OW, Kaufman S, Keller PJ, Kiefer JR, Lai T, Li Y, Liao J, Liu W, Lu H, Pardo E, Tsui V, Wang J, Wang Y, Xu Z, Yan F, Yu D, Zawadzke L, Zhu X, Zhu X, Sims RJ, Cochran AG, Bellon S, Audia JE, Magnuson S, Albrecht BK.
ACS Med Chem Lett (2016) 7 : 531 -536
PubMed 27190605 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 5.89 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3814752 1 7.52
CHEMBL3814550 1 7.48
CHEMBL3814896 1 7.41
CHEMBL3813929 1 7.11
CHEMBL3813846 1 6.96
CHEMBL3814246 1 6.89
CHEMBL3815023 1 6.80
CHEMBL3814197 1 6.62
CHEMBL3814602 1 6.60
CHEMBL3814891 1 6.28
CHEMBL3813915 1 6.21
CHEMBL3814881 1 6.17
CHEMBL3813713 1 6.05
CHEMBL3813731 1 5.72
CHEMBL3814595 1 5.47
CHEMBL3814772 1 5.39
CHEMBL3813744 1 5.30
CHEMBL3815086 1 5.19
CHEMBL3813884 1 5.16
CHEMBL2282031 1 5.00
CHEMBL3814217 1 4.92
CHEMBL3814920 1 4.57
CHEMBL2142704 1 4.50
CHEMBL3813701 1 3.92
CHEMBL3815068 1 3.89
CHEMBL3813958 1 -
CHEMBL3813890 1 -
CHEMBL3814945 1 -
CHEMBL3814706 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3814752 IC50 = 30.0 nM 7.52
CHEMBL3814550 IC50 = 33.0 nM 7.48
CHEMBL3814896 IC50 = 39.0 nM 7.41
CHEMBL3813929 IC50 = 77.0 nM 7.11
CHEMBL3813846 IC50 = 110.0 nM 6.96
CHEMBL3814246 IC50 = 130.0 nM 6.89
CHEMBL3815023 IC50 = 160.0 nM 6.80
CHEMBL3814197 IC50 = 240.0 nM 6.62
CHEMBL3814602 IC50 = 250.0 nM 6.60
CHEMBL3814891 IC50 = 530.0 nM 6.28
CHEMBL3813915 IC50 = 620.0 nM 6.21
CHEMBL3814881 IC50 = 670.0 nM 6.17
CHEMBL3813713 IC50 = 900.0 nM 6.05
CHEMBL3813731 IC50 = 1900.0 nM 5.72
CHEMBL3814595 IC50 = 3400.0 nM 5.47
CHEMBL3814772 IC50 = 4100.0 nM 5.39
CHEMBL3813744 IC50 = 5000.0 nM 5.30
CHEMBL3815086 IC50 = 6500.0 nM 5.19
CHEMBL3813884 IC50 = 7000.0 nM 5.16
CHEMBL2282031 IC50 = 10000.0 nM 5.00
CHEMBL3814217 IC50 = 12000.0 nM 4.92
CHEMBL3814920 IC50 = 27000.0 nM 4.57
CHEMBL2142704 IC50 = 32000.0 nM 4.50
CHEMBL3813701 IC50 = 120000.0 nM 3.92
CHEMBL3815068 IC50 = 130000.0 nM 3.89
CHEMBL3813958 IC50 > 200000.0 nM -
CHEMBL3813890 IC50 > 200000.0 nM -
CHEMBL3814945 IC50 > 200000.0 nM -
CHEMBL3814706 IC50 > 2000000.0 nM -