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Assay Detail

CHEMBL3817161

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Halo-tagged histone H3.3 binding to CBP (unknown origin) expressed in HEK293 cells after overnight incubation by luciferase reporter gene based BRET assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

CREB-binding protein (CHEMBL5747)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Fragment-Based Discovery of a Selective and Cell-Active Benzodiazepinone CBP/EP300 Bromodomain Inhibitor (CPI-637).
Taylor AM, Côté A, Hewitt MC, Pastor R, Leblanc Y, Nasveschuk CG, Romero FA, Crawford TD, Cantone N, Jayaram H, Setser J, Murray J, Beresini MH, de Leon Boenig G, Chen Z, Conery AR, Cummings RT, Dakin LA, Flynn EM, Huang OW, Kaufman S, Keller PJ, Kiefer JR, Lai T, Li Y, Liao J, Liu W, Lu H, Pardo E, Tsui V, Wang J, Wang Y, Xu Z, Yan F, Yu D, Zawadzke L, Zhu X, Zhu X, Sims RJ, Cochran AG, Bellon S, Audia JE, Magnuson S, Albrecht BK.
ACS Med Chem Lett (2016) 7 : 531 -536
PubMed 27190605 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 15 5.59 6.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3814752 1 6.52
CHEMBL3814896 1 5.92
CHEMBL3813929 1 5.72
CHEMBL3814550 1 5.52
CHEMBL3815023 1 5.47
CHEMBL3814246 1 5.30
CHEMBL3814602 1 5.16
CHEMBL3814197 1 5.09
CHEMBL3814595 1 -
CHEMBL3813846 1 -
CHEMBL3814881 1 -
CHEMBL3813915 1 -
CHEMBL3815086 1 -
CHEMBL3813744 1 -
CHEMBL3813713 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3814752 EC50 = 300.0 nM 6.52
CHEMBL3814896 EC50 = 1200.0 nM 5.92
CHEMBL3813929 EC50 = 1900.0 nM 5.72
CHEMBL3814550 EC50 = 3000.0 nM 5.52
CHEMBL3815023 EC50 = 3400.0 nM 5.47
CHEMBL3814246 EC50 = 5000.0 nM 5.30
CHEMBL3814602 EC50 = 7000.0 nM 5.16
CHEMBL3814197 EC50 = 8100.0 nM 5.09
CHEMBL3814595 EC50 > 10000.0 nM -
CHEMBL3813846 EC50 > 10000.0 nM -
CHEMBL3814881 EC50 > 10000.0 nM -
CHEMBL3813915 EC50 > 10000.0 nM -
CHEMBL3815086 EC50 > 10000.0 nM -
CHEMBL3813744 EC50 > 10000.0 nM -
CHEMBL3813713 EC50 > 10000.0 nM -