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Assay Detail

CHEMBL3820005

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of fluorescein-labeled Bid BH3 peptide (79 to 99 residues) binding to His-tagged MCL1 (171 to 327 residues) (unknown origin) expressed in Escherichia coli Bl21(DE3) cells incubated for 3 hrs by fluorescence polarization assay
4
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Small molecules targeting Mcl-1: the search for a silver bullet in cancer therapy
Nhu D, Lessene G, Huang DCS, Burns CJ
Medchemcomm (2016) 7 : 778 -787
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 6.77 6.77
Ki 1 5.73 5.73

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3265300 1 6.77
CHEMBL3265297 1 6.77
CHEMBL3265298 1 6.77
CHEMBL1331211 1 5.73

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3265300 IC50 = 170.0 nM 6.77
CHEMBL3265297 IC50 = 170.0 nM 6.77
CHEMBL3265298 IC50 = 170.0 nM 6.77
CHEMBL1331211 Ki = 1870.0 nM 5.73