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Assay Detail

CHEMBL3820409

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of bovine recombinant eNOS expressed in Escherichia coli using L-arginine as substrate assessed as reduction in NO production measured for 6 mins in presence of tetrahydrobiopterin by hemoglobin capture assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nitric oxide synthase 3 (CHEMBL4802)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Potent and Selective Human Neuronal Nitric Oxide Synthase Inhibition by Optimization of the 2-Aminopyridine-Based Scaffold with a Pyridine Linker.
Wang HY, Qin Y, Li H, Roman LJ, Martásek P, Poulos TL, Silverman RB.
J Med Chem (2016) 59 : 4913 -4925
PubMed 27050842 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 4.78 4.88

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3818373 1 4.88
CHEMBL3818566 1 4.78
CHEMBL3819551 1 4.69
CHEMBL3818545 1 -
CHEMBL3819582 1 -
CHEMBL3818649 1 -
CHEMBL3819644 1 -
CHEMBL3818073 1 -
CHEMBL3819046 1 -
CHEMBL3818486 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3818373 Ki = 13247.0 nM 4.88
CHEMBL3818566 Ki = 16637.0 nM 4.78
CHEMBL3819551 Ki = 20487.0 nM 4.69
CHEMBL3818545 Ki > 30000.0 nM -
CHEMBL3819582 Ki - -
CHEMBL3818649 Ki - -
CHEMBL3819644 Ki - -
CHEMBL3818073 Ki - -
CHEMBL3819046 Ki - -
CHEMBL3818486 Ki - -