Assay Detail
Binding
CHEMBL3820552
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to GST-fused human carbonic anhydrase-2 expressed in Escherichia coli BL21 codon plus cells assessed as dissociation constant by SPR method
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Kinetic and Structural Insights into the Mechanism of Binding of Sulfonamides to Human Carbonic Anhydrase by Computational and Experimental Studies.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 7 | 4.89 | 6.05 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL414 | CARZENIDE | 2.0 | 1 | 6.05 |
| CHEMBL21 | SULFANILAMIDE | 4.0 | 1 | 5.26 |
| CHEMBL148660 | — | — | 1 | 4.24 |
| CHEMBL358263 | — | — | 1 | 4.01 |
| CHEMBL27601 | BENZENESULFONAMIDE | — | 1 | - |
| CHEMBL574 | P-TOLUENESULFONAMIDE | 3.0 | 1 | - |
| CHEMBL6784 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL414 | CARZENIDE | Kd | = | 890.0 | nM | 6.05 |
| CHEMBL21 | SULFANILAMIDE | Kd | = | 5550.0 | nM | 5.26 |
| CHEMBL148660 | — | Kd | = | 57800.0 | nM | 4.24 |
| CHEMBL358263 | — | Kd | = | 98300.0 | nM | 4.01 |
| CHEMBL27601 | BENZENESULFONAMIDE | Kd | = | 1149700.0 | nM | - |
| CHEMBL574 | P-TOLUENESULFONAMIDE | Kd | = | 292900.0 | nM | - |
| CHEMBL6784 | — | Kd | = | 392000.0 | nM | - |