Assay Detail
Binding
CHEMBL3822089
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Binding affinity to His8-tagged Lactococcus lactis ThiT expressed in Lactococcus lactis NZ9000 cells by intrinsic fluorescence titration assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Lactococcus lactis |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Design and synthesis of thiamine analogues to study their binding to the ECF transporter for thiamine in bacteria
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 6 | 8.09 | 9.91 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1588 | THIAMINE | 4.0 | 1 | 9.91 |
| CHEMBL401772 | 3-DEAZATHIAMINE | — | 1 | 8.37 |
| CHEMBL3818038 | — | — | 1 | 8.30 |
| CHEMBL401572 | — | — | 1 | 8.07 |
| CHEMBL255550 | — | — | 1 | 7.87 |
| CHEMBL257285 | — | — | 1 | 5.99 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1588 | THIAMINE | Kd | = | 0.122 | nM | 9.91 |
| CHEMBL401772 | 3-DEAZATHIAMINE | Kd | = | 4.23 | nM | 8.37 |
| CHEMBL3818038 | — | Kd | = | 5.04 | nM | 8.30 |
| CHEMBL401572 | — | Kd | = | 8.48 | nM | 8.07 |
| CHEMBL255550 | — | Kd | = | 13.58 | nM | 7.87 |
| CHEMBL257285 | — | Kd | = | 1025.0 | nM | 5.99 |