Assay Detail
Binding
CHEMBL3822337
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of FITC-labeled Bid BH3 peptide binding to human GST-tagged BCL2 lacking C-terminal transmembrane domain expressed in Escherichia coli XL-1 Blue cells preincubated for 10 mins followed by FITC-labeled Bid BH3 peptide addition measured after 20 mins by fluorescence polarization assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | XL-1 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Small molecules targeting Mcl-1: the search for a silver bullet in cancer therapy
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 5.96 | 5.96 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL408194 | OBATOCLAX | 3.0 | 1 | 5.96 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL408194 | OBATOCLAX | IC50 | = | 1110.0 | nM | 5.96 |