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Assay Detail

CHEMBL3822339

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of FITC-labeled Bim BH3 peptide binding to GST-tagged BCL2 (unknown origin) preincubated for 2 mins followed by FITC-labeled Bim BH3 peptide addition measured after 10 mins by fluorescence polarization assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Apoptosis regulator Bcl-2 (CHEMBL4860)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Small molecules targeting Mcl-1: the search for a silver bullet in cancer therapy
Nhu D, Lessene G, Huang DCS, Burns CJ
Medchemcomm (2016) 7 : 778 -787
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 1 5.55 5.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL551288 APOGOSSYPOL 1 5.55

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL551288 APOGOSSYPOL Ki = 2800.0 nM 5.55