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Assay Detail

CHEMBL3824531

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of C-terminal His-tagged full length human SRC expressed in insect cells preincubated for 20 mins using poly[Glu,Tyr]4:1 as substrate measured after 5 to 120 mins in presence of [gamma-33P]ATP by Kinome assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proto-oncogene tyrosine-protein kinase Src (CHEMBL267)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Rapid Discovery and Structure-Activity Relationships of Pyrazolopyrimidines That Potently Suppress Breast Cancer Cell Growth via SRC Kinase Inhibition with Exceptional Selectivity over ABL Kinase.
Fraser C, Dawson JC, Dowling R, Houston DR, Weiss JT, Munro AF, Muir M, Harrington L, Webster SP, Frame MC, Brunton VG, Patton EE, Carragher NO, Unciti-Broceta A.
J Med Chem (2016) 59 : 4697 -4710
PubMed 27115835 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.47 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3823104 1 9.22
CHEMBL3824116 1 9.00
CHEMBL3823620 1 9.00
CHEMBL3823543 1 8.46
CHEMBL306380 1 7.77
CHEMBL3823451 1 7.68
CHEMBL3823861 1 7.57
CHEMBL3824228 1 7.15
CHEMBL3823255 1 6.94
CHEMBL3823212 1 6.90
CHEMBL3823711 1 6.71
CHEMBL3823849 1 6.52
CHEMBL3822591 1 5.98
CHEMBL3799221 1 5.61
CHEMBL3824233 1 -
CHEMBL3824089 1 -
CHEMBL3824021 1 -
CHEMBL5416410 DASATINIB 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3823104 IC50 = 0.6 nM 9.22
CHEMBL3824116 IC50 = 1.0 nM 9.00
CHEMBL3823620 IC50 = 1.0 nM 9.00
CHEMBL3823543 IC50 = 3.5 nM 8.46
CHEMBL306380 IC50 = 17.0 nM 7.77
CHEMBL3823451 IC50 = 21.0 nM 7.68
CHEMBL3823861 IC50 = 27.0 nM 7.57
CHEMBL3824228 IC50 = 71.0 nM 7.15
CHEMBL3823255 IC50 = 114.0 nM 6.94
CHEMBL3823212 IC50 = 126.0 nM 6.90
CHEMBL3823711 IC50 = 194.0 nM 6.71
CHEMBL3823849 IC50 = 303.0 nM 6.52
CHEMBL3822591 IC50 = 1040.0 nM 5.98
CHEMBL3799221 IC50 = 2453.0 nM 5.61
CHEMBL3824233 IC50 < 0.5 nM -
CHEMBL3824089 IC50 < 0.5 nM -
CHEMBL3824021 IC50 > 10000.0 nM -
CHEMBL5416410 DASATINIB IC50 < 0.5 nM -