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Assay Detail

CHEMBL3825096

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human iGluA1 receptor flop isoform expressed in CHO-S cells coexpressing TARP gamma-4 assessed as inhibition of glutamate-induced increase in intracellular calcium levels after 2 mins followed by cyclothiazide/glutamate addition by fluorescence imaging plate reader method
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-S
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glutamate receptor 1 (CHEMBL2009)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of the First α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Antagonist Dependent upon Transmembrane AMPA Receptor Regulatory Protein (TARP) γ-8.
Gardinier KM, Gernert DL, Porter WJ, Reel JK, Ornstein PL, Spinazze P, Stevens FC, Hahn P, Hollinshead SP, Mayhugh D, Schkeryantz J, Khilevich A, De Frutos O, Gleason SD, Kato AS, Luffer-Atlas D, Desai PV, Swanson S, Burris KD, Ding C, Heinz BA, Need AB, Barth VN, Stephenson GA, Diseroad BA, Woods TA, Yu H, Bredt D, Witkin JM.
J Med Chem (2016) 59 : 4753 -4768
PubMed 27067148 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.21 6.21

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1214124 PERAMPANEL 4.0 1 6.21
CHEMBL3823441 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1214124 PERAMPANEL IC50 = 623.0 nM 6.21
CHEMBL3823441 IC50 > 83300.0 nM -