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Assay Detail

CHEMBL3828962

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition full length human recombinant HDAC2 expressed in baculovirus coexpressed in fall armyworm Sf9 cells using carboxyfluorescein (FAM)-labeled acetylated/ trifluoroacetylated peptide as substrate preincubated for 3 hrs measured after 60 mins by fluorescence assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 2 (CHEMBL1937)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Kinetic and structural insights into the binding of histone deacetylase 1 and 2 (HDAC1, 2) inhibitors.
Wagner FF, Weïwer M, Steinbacher S, Schomburg A, Reinemer P, Gale JP, Campbell AJ, Fisher SL, Zhao WN, Reis SA, Hennig KM, Thomas M, Müller P, Jefson MR, Fass DM, Haggarty SJ, Zhang YL, Holson EB.
Bioorg Med Chem (2016) 24 : 4008 -4015
PubMed 27377864 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.89 7.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL235842 1 7.89

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL235842 IC50 = 13.0 nM 7.89