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Assay Detail

CHEMBL3830163

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Staphylococcus aureus ATCC 27659 full length His-tagged dehydrosqualene synthase expressed in Escherichia coli BL21 (DE3) cells assessed as reduction in staphyloxanthin pigment formation after 72 hrs by spectrophotometric analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Staphylococcus aureus
Cell Type BL21-DE3
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

4,4'-diapophytoene synthase (CHEMBL5440)
Type SINGLE PROTEIN
Organism Staphylococcus aureus

Publication

Discovery of Benzocycloalkane Derivatives Efficiently Blocking Bacterial Virulence for the Treatment of Methicillin-Resistant S. aureus (MRSA) Infections by Targeting Diapophytoene Desaturase (CrtN).
Wang Y, Di H, Chen F, Xu Y, Xiao Q, Wang X, Wei H, Lu Y, Zhang L, Zhu J, Lan L, Li J.
J Med Chem (2016) 59 : 4831 -4848
PubMed 27139780 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.08 7.08

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL351151 1 7.08

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL351151 IC50 = 83.4 nM 7.08