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Assay Detail

CHEMBL3853686

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Non-competitive inhibition of jack bean beta-N-acetylglucosaminidase using p-nitrophenyl-N-acetyl-beta-D-glucosamide as substrate at pH 5.5 incubated for 10 to 30 mins by Linerweaver-Burk plot/double reciprocal plot analysis
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Canavalia ensiformis
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Efficient stereoselective synthesis of 2-acetamido-1,2-dideoxyallonojirimycin (DAJNAc) and sp(2)-iminosugar conjugates: Novel hexosaminidase inhibitors with discrimination capabilities between the mature and precursor forms of the enzyme.
de la Fuente A, Rísquez-Cuadro R, Verdaguer X, García Fernández JM, Nanba E, Higaki K, Ortiz Mellet C, Riera A.
Eur J Med Chem (2016) 121 : 926 -938
PubMed 26564401 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 4.94 5.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3983808 1 5.64
CHEMBL3966025 1 5.58
CHEMBL3932594 1 5.02
CHEMBL3895004 1 4.87
CHEMBL3982827 1 4.70
CHEMBL3955165 1 4.63
CHEMBL3936181 1 4.12
CHEMBL3933402 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3983808 Ki = 2300.0 nM 5.64
CHEMBL3966025 Ki = 2600.0 nM 5.58
CHEMBL3932594 Ki = 9500.0 nM 5.02
CHEMBL3895004 Ki = 13400.0 nM 4.87
CHEMBL3982827 Ki = 20000.0 nM 4.70
CHEMBL3955165 Ki = 23600.0 nM 4.63
CHEMBL3936181 Ki = 75000.0 nM 4.12
CHEMBL3933402 Ki = 363000.0 nM -