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Assay Detail

CHEMBL3853690

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of beta-hexosaminidase alpha in human skin fibroblast lysate using 4-MU-N-acetyl-beta-D-glucosaminide-6-sulfate sodium salt as substrate assessed as 4-MU liberation by fluorescence assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-hexosaminidase subunit alpha (CHEMBL1250415)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Efficient stereoselective synthesis of 2-acetamido-1,2-dideoxyallonojirimycin (DAJNAc) and sp(2)-iminosugar conjugates: Novel hexosaminidase inhibitors with discrimination capabilities between the mature and precursor forms of the enzyme.
de la Fuente A, Rísquez-Cuadro R, Verdaguer X, García Fernández JM, Nanba E, Higaki K, Ortiz Mellet C, Riera A.
Eur J Med Chem (2016) 121 : 926 -938
PubMed 26564401 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.00 4.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3983808 1 4.00
CHEMBL3982827 1 4.00
CHEMBL3933402 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3983808 IC50 = 100000.0 nM 4.00
CHEMBL3982827 IC50 = 100000.0 nM 4.00
CHEMBL3933402 IC50 = 350000.0 nM -