Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3854595

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Pseudomonas aeruginosa HemO mutant expressed in Escherichia coli BL21(DE3) assessed as decrease in biliverdin-9alpha-dependent IFP1.4 fluorescence after 16 hrs by spectrophotometric method
26
Total Activities
25
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Pseudomonas aeruginosa
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Iminoguanidines as Allosteric Inhibitors of the Iron-Regulated Heme Oxygenase (HemO) of Pseudomonas aeruginosa.
Heinzl GA, Huang W, Yu W, Giardina BJ, Zhou Y, MacKerell AD, Wilks A, Xue F.
J Med Chem (2016) 59 : 6929 -6942
PubMed 27353344 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 25 4.47 4.95
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2346953 1 4.95
CHEMBL3960464 1 4.95
CHEMBL2346945 1 4.92
CHEMBL2346944 1 4.71
CHEMBL3982697 1 4.69
CHEMBL3920262 1 4.66
CHEMBL3889940 1 4.46
CHEMBL3900440 1 4.45
CHEMBL1976834 1 4.45
CHEMBL2346937 1 4.37
CHEMBL3969135 1 4.35
CHEMBL2346938 1 4.28
CHEMBL3932345 1 4.22
CHEMBL104315 1 4.19
CHEMBL105099 1 4.14
CHEMBL2346941 1 4.11
CHEMBL2346950 1 4.11
CHEMBL3944104 1 -
CHEMBL3975335 1 -
CHEMBL3928103 1 -
CHEMBL3955732 1 -
CHEMBL105167 2 -
CHEMBL105616 1 -
CHEMBL54151 1 -
CHEMBL2346942 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2346953 EC50 = 11300.0 nM 4.95
CHEMBL3960464 EC50 = 11200.0 nM 4.95
CHEMBL2346945 EC50 = 11900.0 nM 4.92
CHEMBL2346944 EC50 = 19400.0 nM 4.71
CHEMBL3982697 EC50 = 20300.0 nM 4.69
CHEMBL3920262 EC50 = 21900.0 nM 4.66
CHEMBL3889940 EC50 = 34300.0 nM 4.46
CHEMBL3900440 EC50 = 35300.0 nM 4.45
CHEMBL1976834 EC50 = 35600.0 nM 4.45
CHEMBL2346937 EC50 = 43100.0 nM 4.37
CHEMBL3969135 EC50 = 45100.0 nM 4.35
CHEMBL2346938 EC50 = 52400.0 nM 4.28
CHEMBL3932345 EC50 = 60300.0 nM 4.22
CHEMBL104315 EC50 = 64400.0 nM 4.19
CHEMBL105099 EC50 = 72700.0 nM 4.14
CHEMBL2346950 EC50 = 77400.0 nM 4.11
CHEMBL2346941 EC50 = 77500.0 nM 4.11
CHEMBL105167 Inhibition - % -
CHEMBL3955732 EC50 > 200000.0 nM -
CHEMBL3975335 EC50 - -
CHEMBL3928103 EC50 > 200000.0 nM -
CHEMBL105167 EC50 = 123000.0 nM -
CHEMBL3944104 EC50 > 200000.0 nM -
CHEMBL105616 EC50 = 102000.0 nM -
CHEMBL54151 EC50 = 122000.0 nM -
CHEMBL2346942 EC50 > 200000.0 nM -