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Assay Detail

CHEMBL3854636

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant bovine GRK3 expressed in Sf9 insect cells assessed as decrease in phosphorylation of urea-washed bovine rod outer segments in presence of Gbetagamma subunits and [gamma-32P]-ATP after 5 to 60 mins by SDS-PAGE analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G protein-coupled receptor kinase 3 (CHEMBL3711487)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

G-Protein-Coupled Receptor Kinase 2 (GRK2) Inhibitors: Current Trends and Future Perspectives.
Guccione M, Ettari R, Taliani S, Da Settimo F, Zappalà M, Grasso S.
J Med Chem (2016) 59 : 9277 -9294
PubMed 27362616 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 4.90 5.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3950887 1 5.82
CHEMBL3931500 1 5.21
CHEMBL3942164 1 4.94
CHEMBL3953837 1 4.65
CHEMBL3900757 1 4.52
CHEMBL3971763 1 4.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3950887 IC50 = 1500.0 nM 5.82
CHEMBL3931500 IC50 = 6100.0 nM 5.21
CHEMBL3942164 IC50 = 11600.0 nM 4.94
CHEMBL3953837 IC50 = 22600.0 nM 4.65
CHEMBL3900757 IC50 = 30400.0 nM 4.52
CHEMBL3971763 IC50 = 55500.0 nM 4.26