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Assay Detail

CHEMBL3854659

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human HK1 using H-D-Val-Leu-Arg-AFC as substrate assessed as release of AFC after 10 to 120 mins by spectrofluorimetric method
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kallikrein-1 (CHEMBL2319)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Highly Potent, Selective, and Orally Bioavailable Macrocyclic Inhibitor of Blood Coagulation Factor VIIa-Tissue Factor Complex.
Zhang X, Glunz PW, Johnson JA, Jiang W, Jacutin-Porte S, Ladziata V, Zou Y, Phillips MS, Wurtz NR, Parkhurst B, Rendina AR, Harper TM, Cheney DL, Luettgen JM, Wong PC, Seiffert D, Wexler RR, Priestley ES.
J Med Chem (2016) 59 : 7125 -7137
PubMed 27455395 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 6.53 7.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3907376 1 7.68
CHEMBL3935309 1 7.47
CHEMBL3909009 1 7.00
CHEMBL3951940 1 6.70
CHEMBL3942944 1 6.58
CHEMBL3891120 1 6.57
CHEMBL3980787 1 6.41
CHEMBL3926417 1 6.24
CHEMBL3917434 1 6.22
CHEMBL3902498 1 6.19
CHEMBL3930523 1 6.19
CHEMBL3901391 1 6.10
CHEMBL3978562 1 5.58

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3907376 Ki = 21.0 nM 7.68
CHEMBL3935309 Ki = 34.0 nM 7.47
CHEMBL3909009 Ki = 100.0 nM 7.00
CHEMBL3951940 Ki = 200.0 nM 6.70
CHEMBL3942944 Ki = 260.0 nM 6.58
CHEMBL3891120 Ki = 270.0 nM 6.57
CHEMBL3980787 Ki = 390.0 nM 6.41
CHEMBL3926417 Ki = 580.0 nM 6.24
CHEMBL3917434 Ki = 600.0 nM 6.22
CHEMBL3902498 Ki = 650.0 nM 6.19
CHEMBL3930523 Ki = 650.0 nM 6.19
CHEMBL3901391 Ki = 790.0 nM 6.10
CHEMBL3978562 Ki = 2600.0 nM 5.58