Assay Detail
Binding
CHEMBL3854711
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to recombinant human AMPK alpha1/beta1/gamma1 by SPR binding assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Target
AMP-activated protein kinase (AMPK) alpha-1/beta-1/gamma-1 (CHEMBL2111345) ↗| Type | PROTEIN COMPLEX |
| Organism | Homo sapiens |
Publication
Discovery and Preclinical Characterization of 6-Chloro-5-[4-(1-hydroxycyclobutyl)phenyl]-1H-indole-3-carboxylic Acid (PF-06409577), a Direct Activator of Adenosine Monophosphate-activated Protein Kinase (AMPK), for the Potential Treatment of Diabetic Nephropathy.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 7 | 6.53 | 8.05 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3891221 | — | — | 1 | 8.05 |
| CHEMBL3893781 | — | — | 1 | 7.64 |
| CHEMBL3917068 | — | — | 1 | 7.04 |
| CHEMBL3919304 | — | — | 1 | 6.82 |
| CHEMBL3950160 | — | — | 1 | 5.75 |
| CHEMBL752 | ADENOSINE PHOSPHATE | 4.0 | 1 | 5.43 |
| CHEMBL3936376 | — | — | 1 | 5.00 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3891221 | — | Kd | = | 9.0 | nM | 8.05 |
| CHEMBL3893781 | — | Kd | = | 22.7 | nM | 7.64 |
| CHEMBL3917068 | — | Kd | = | 90.2 | nM | 7.04 |
| CHEMBL3919304 | — | Kd | = | 149.8 | nM | 6.82 |
| CHEMBL3950160 | — | Kd | = | 1776.0 | nM | 5.75 |
| CHEMBL752 | ADENOSINE PHOSPHATE | Kd | = | 3700.0 | nM | 5.43 |
| CHEMBL3936376 | — | Kd | = | 9948.0 | nM | 5.00 |