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Assay Detail

CHEMBL3855407

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at LXRalpha LBD (unknown origin) fused with Gal4-DNA binding domain expressed in HEK293 cells assessed as modulation of receptor transcription activity by luciferase reporter gene assay
16
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Oxysterols receptor LXR-alpha (CHEMBL2808)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Brain penetrant liver X receptor (LXR) modulators based on a 2,4,5,6-tetrahydropyrrolo[3,4-c]pyrazole core.
Tice CM, Noto PB, Fan KY, Zhao W, Lotesta SD, Dong C, Marcus AP, Zheng YJ, Chen G, Wu Z, Van Orden R, Zhou J, Bukhtiyarov Y, Zhao Y, Lipinski K, Howard L, Guo J, Kandpal G, Meng S, Hardy A, Krosky P, Gregg RE, Leftheris K, McKeever BM, Singh SB, Lala D, McGeehan GM, Zhuang L, Claremon DA.
Bioorg Med Chem Lett (2016) 26 : 5044 -5050
PubMed 27599745 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 6.66 7.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3905741 1 7.43
CHEMBL3914727 1 7.24
CHEMBL3985591 1 7.14
CHEMBL3976470 1 7.03
CHEMBL3959681 1 6.96
CHEMBL3940521 1 6.78
CHEMBL3913348 1 6.72
CHEMBL3960195 1 6.70
CHEMBL3978980 1 6.67
CHEMBL3814206 1 6.61
CHEMBL3932169 1 6.60
CHEMBL3956923 1 6.57
CHEMBL3972392 1 6.31
CHEMBL3933543 1 6.25
CHEMBL3889601 2 5.86

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3905741 EC50 = 37.0 nM 7.43
CHEMBL3914727 EC50 = 57.0 nM 7.24
CHEMBL3985591 EC50 = 72.0 nM 7.14
CHEMBL3976470 EC50 = 94.0 nM 7.03
CHEMBL3959681 EC50 = 111.0 nM 6.96
CHEMBL3940521 EC50 = 166.0 nM 6.78
CHEMBL3913348 EC50 = 189.0 nM 6.72
CHEMBL3960195 EC50 = 199.0 nM 6.70
CHEMBL3978980 EC50 = 214.0 nM 6.67
CHEMBL3814206 EC50 = 244.0 nM 6.61
CHEMBL3932169 EC50 = 249.0 nM 6.60
CHEMBL3956923 EC50 = 271.0 nM 6.57
CHEMBL3972392 EC50 = 491.0 nM 6.31
CHEMBL3933543 EC50 = 567.0 nM 6.25
CHEMBL3889601 EC50 = 1376.0 nM 5.86
CHEMBL3889601 EC50 = 2241.0 nM 5.65